Hi, Theo,

Kasumi-1 cell line takes 3-4 weeks to be on exponential growth, and then your doubling time is around 36 hours.

Best regards.

Thanks for the answers.

The cells are growing decently well.

At this point I am looking to start pushing them to differentiate with different cytokine cocktails. Wondering if anyone has done this already and has found any major areas to troubleshoot.

Hi Sandra,

I am suffering the decrease in viability that you described for kasumi-1. It is the second time that kasumi-1 cells finally died after two weeks of culture. What is your recommendation to deal with this problem?

Hi Carla Carla Ijurko,

I have the same problem. Did you find a solution to it? Is it true that you need to have them in culture for several weeks before they start to expand? With other cell lines I always tried to keep the passage number as low as possible. I tried with 10% FBS containing media RPMI, 20% FBS in RPMI and with and without sodium pyruvate. I am thankful for any help!