What is the Doubling Time of Kasumi-1 & KG-1 AML Cells?

More Theo Borgovan's questions See All

How to calculate electrical conductivity and charge carriers density with DOS and Transmission?

Hi everyone, I'm trying to simulate some graphene nanoribbons to determine their electron mobility. I use DFTB+ for simulation software. With this software I can plot transmission in regards of...

03 February 2021 9,315 3 View

How to find molecular energy transition from given chemiluminescent emission wavelength?

Hello, As part of my master's thesis, we are gasifying biomass in an entrained flow reactor. We are collecting the light emitted by the flame via a collimating lens and an optical fibre in order...

09 September 2020 1,262 2 View

What are the precise roles of Machine learning in Drug discovery?

Does anyone know what the end-goal of developing artificial intelligence for aiding the drug discovery process is? How would that theoretically work?

09 April 2019 1,642 10 View

Does someone has a pdf of?

31 May 2016 2,320 7 View

Can anybody help me to obtain pure saponite and hectorite samples?

For detailed characterization by XPS and MAS-NMR, I am looking for a series of phase pure saponite and hectorite samples from around the world of about a gram per sample. Both natural and...

04 July 2014 3,275 4 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

Can I arbitrarily take a cycle number of 40 for the gene that was not expressed at all in qPCR results calculation?

I ran RT-qPCR for a control and a treated group and it appears that my gene of interest was not expressed at all in any of the subjects in the control group. So I was wondering if I could...

20 February 2021 6,243 10 View

Does anyone know if the Quantstudio 3 qPCR machine requires a passive reference when Using SYBR? I ran a qPCR and got the plot in the attached file.?

I know that my target is in my sample as I extracted it from cell culture myself. These plots show some amplification. The first one seems O.K for the first 36 cycles but then collapses at the...

14 February 2021 2,809 2 View

Are there any exception like work hardening in metals elastic limit due to dynamic loading?

The samples made out of spring steel are taken from same lot. The specimens were shaped following heating to forging temperature - Forming- Hardening -Tempering methods. After storage of almost 6...

11 February 2021 952 6 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View

ACE2 Blotted as a double band?

Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...

07 February 2021 6,973 3 View

Having trouble with qPCR amplification plots. My curve's never seem to reach a plateau. Any ideas?

The parameters of the curve all seem acceptable, typically reactions results are above 0.9852, -3.1 to -3.6 slope, and 90-110% reaction efficiency. The results seem to be what I would expect from...

04 February 2021 1,138 3 View

Queries regarding the rain flow counting algorithm?

02 February 2021 9,790 2 View

What is the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow endothelial cells?

Hello I am new to work on endothelial cells, and I have been reading about them a lot and trying to know the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow...

28 January 2021 8,765 4 View

How to gate mouse MDSC cells through flow cytometry?

On inducing the mouse bone marrow cells with X compound, there is a change in cells morphology and characteristics. In that case how to gate these cells, as these cells are showing suppressive...

27 January 2021 608 2 View

Juan Coelho-Silva

Hi, Theo,

Kasumi-1 cell line takes 3-4 weeks to be on exponential growth, and then your doubling time is around 36 hours.

Best regards.

Theo Borgovan

Thanks for the answers.

The cells are growing decently well.

At this point I am looking to start pushing them to differentiate with different cytokine cocktails. Wondering if anyone has done this already and has found any major areas to troubleshoot.

Carla Ijurko

Hi Sandra,

I am suffering the decrease in viability that you described for kasumi-1. It is the second time that kasumi-1 cells finally died after two weeks of culture. What is your recommendation to deal with this problem?

Caroline Leijonhufvud

Hi Carla Carla Ijurko,

I have the same problem. Did you find a solution to it? Is it true that you need to have them in culture for several weeks before they start to expand? With other cell lines I always tried to keep the passage number as low as possible. I tried with 10% FBS containing media RPMI, 20% FBS in RPMI and with and without sodium pyruvate. I am thankful for any help!