I'm going to do a wound healing assay with a cytosine arabinoside like proliferation inhibitor, but I don't know if I have to administrate this inhibitor every 24 hours o what time is the best. I'm going to administrate treatment of a protein every 24h and removal media but the question is... I have to administrate de cytosine arabinoside every time I change the media and protein? or just with once administration of cytosine arabinoside is enough?
I'm going to take photos until the 96h
Hello Marian.
Better to check both ways to find the best working of your assay system.
Trial 1> Vehicle alone [(-)ve control], Arab-C addition for 48h (treated), Arab-C addition + protein (healing) and Vehicle alone + protein [healing on (-) control] groups: Arab-C and protein are added once.
Trial 2> Arab-C addition for 48h (treated), then release from Arab-C by complete washing gently. (-)Protein (healing) and (+)protein culture groups for further 48h.
You could set up 24h, too, depending on cell viability test first.
Best wishes.
Hi Marian,
I was once performed drug experiments with Ara-C in leukemia cells. Because leukemia cells are suspended cells, I added Ara-C into culture media every 24 hours within 72 hours. In view of your case, you need to optimize the best time of administration as well as the best working concentration of Ara-c, and determine how often you add it within your setting by carrying out several pilot experiments, as suggested by Dr. Lee above.
- Badges
- Science topic
- Similar topics
- Biological Science
- Cell Biology
- Culture Cells