Clinical Pharmacology

Clinical Pharmacology and Clinical Therapeutics | Review and cite CLINICAL PHARMACOLOGY protocol, troubleshooting and other methodology information | Contact experts in CLINICAL PHARMACOLOGY to get answers

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Questions related to Clinical Pharmacology

What should be the adequate range of cry1Ac protein (ug/g) to kill helicoverpa armigera in bt cotton?

The protein dry wet expression range for leaves/square/bolls parts will be approximately similar or will be different? Is their any standard range?

21 April 2023 1,629 0 View

What chemicals cna I use that do not affect bacterial growth?

I would like to compare the growth of bacteria exposed to a particular chemical to that of something that does not affect their growth. Since I am using the non-exposed at all bacteria to...

12 April 2023 3,708 1 View

How do you calculate a method detection limit for rates in oxygen consumption?

We have collection oxygen consumption measurements using PreSens spot sensors in the PreSens Sensor Dish system and ran a 12 Milli-Q blanks. In this system you can continuously monitor the oxygen...

06 April 2023 9,778 0 View

Hi i want equations for calcium and magnesium calculation in water ?

I have the volume of edta but i need how to calculate calcium and magnesium by mg/l

05 April 2023 5,038 1 View

What is the role of steroids in acute epiglottitis in elderly adults with mild respiratory distress ?

Is there any role for corticosteroids in the setting of management of epiglottitis in elderly adults with mild respiratory distress ?

31 March 2023 5,715 0 View

Should I remove remaining materials in defect site before doing microCT scan and analysis?

Dear colleagues I plan to do microCT scan and analysis of 4 mm cranial defect model in rats. However, I am confused about several technical things. I did the bone defect treatment by using several...

13 February 2023 3,206 2 View

What is better to use to monitor CAF activation? Adult fibroblasts or fetal fibroblasts?

Hi, I have been having difficulties in monitoring CAF activation because the lung fibroblasts Wi-38 that I am using seem to already have high levels of expression of a-SMA, FAP and PDGFRb,...

06 February 2023 6,958 2 View

Has anyone had experience adding nortriptyline to a MAOI? Is it safe? Does it work?

https://www.psychotropical.com/attenuation-maoi-pressor-response-nris/

31 January 2023 5,874 5 View

I am searching for a commercially available RWPE-1 cell line to use as a healthy tissue control for prostate cancer research experiments?

Hello PCa research community, I am searching for an appropriate prostate epithelial cell line to use as a non-cancerous, non-tumorigenic, control for my PCa experiments. I came across the RWPE-1...

22 January 2023 4,698 1 View

Is Quercetin better to Taxifolin i.e. Dihydroquercetin or Isoquercetin as anti inflammatory bioactive molecule?

These are emerging bioactivbioactivees to manage hypertension, heart disease, and arthritis amongst others. It is recognized as "safe' to consume in normal proportions vide USA & EU standards....

17 January 2023 9,448 1 View

Can Halo's disappear?

Hello, For school I'm doing a project on bacteria and plastic degradation. For the method I had a R2A agar plate, then I made a suspension of a bacteria and used a sterile cotton swab to put 5...

13 January 2023 3,481 3 View

Is 6xHis tag compatible with therapeutic (GMP) protein production?

Hello! We are producing a recombinant protein (from mammal cells) and we need a 6xHis tag for the purification. This protein is aim to be used in preclinical experiments in the short-medium term,...

12 January 2023 618 2 View

What is the simplest method for hematocrit correction during glucose measurement in electrochemical dry strip technology?

Hematocrit correction during glucose measurement is very important for enhancing the accuracy of measurement in whole blood. As far my knowledge, it done electrochemically either through impedance...

21 December 2022 8,573 0 View

What should we do to resolve the problem of formation of metabolites and disintegration of drug during drug release application of hydrogel ?

We are working on polysaccharide based hydrogel (containing high vitamin C), on its drug (Paracetamol also we used Methotrexate salt) release studies using phosphate buffer as a solvent it is...

08 December 2022 7,222 2 View

Does the solution of the Cahn-Hilliard Equation satisfy or fail to satisfy the maximum principle?

The Cahn-Hilliard equation is a fourth-order nonlinear stiff partial differential equation describing the phase separation process by which the two components of a binary fluid spontaneously...

02 December 2022 2,229 0 View

Is necessary to use a buffer to rinse the samples stained with DAPI to detecte phytoplasma?

I want to stain a phytoplasma in the phloem of infected plant samples previously fixed in FAA, washed in distilled water and cut freehand. In general, all DAPI techniques rinse with a buffer...

18 November 2022 5,100 0 View

How to prevent spreading depression in mouse visual cortex slices?

I'm looking for tips on how to prevent spreading depression that has been repeatedly occurring in my mouse visual cortex slices. I've been washing out Mg2+ to induce low frequency oscillations in...

10 November 2022 6,873 0 View

Could you give me a recommandation for surface LDLR detection by flow cytometry?

I'm looking at surface LDLR expression in huh7 cells by flow cytometry. As known as, sterol deficiency-induced LDLR up-regulation. Therefore, the cells were treated with 5% LPDS for 24 hours. And...

03 November 2022 905 0 View

How to standerdize Mito-SPY protocol on live cells?

Hello everyone! I have an urgent question. I have observed mitochondrial-genes downregulation (RNA-seq). I performed Mito-spy staining on cultured Schwann cells from wild type and can see proper...

01 November 2022 1,374 0 View

What might be the reason Pseudomonas pyocyanin production not evident on nutrient agar?

Pseudomonas aeruginosa is not showing pigmentation when incubated at 37 degree centrigrade for 48 hours on nutrient agar plate. Is any component of the media responsible for it?

25 October 2022 9,831 0 View

Best statistical way to determine vitamin deficiency?

Hello Guys I have collected 9000 vitamin D samples and I want to know how to determine how many of the samples (having Vitamin D deficiency, Insufficiency, Normal, or toxicity). what I mean is how...

17 October 2022 9,759 1 View

What is the purpose of transplanting leukemic cells into healthy mice?

During my research about AML I read a lot about transplanting leukemic cells from mutated mice into healthy mice to see if they develope leukemic phenotype or not. What does it say about the...

11 October 2022 698 0 View

What causes the medium pH to drop in aureobasidium pullulans?

The pH of the medium decreases in 30 hours of aureobasidium pullulans

29 September 2022 9,718 0 View

Are there any clinical trials accepting candidates for a study on the use of psilocybin mushrooms for depression in North Carolina?

A client's depression has not been alleviated although she has been completely compliant with treatment. She has no family history of other mental illnesses. She has tried pharmaceuticals (Prozac,...

25 August 2022 3,242 3 View

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