Clinical Pharmacology

Clinical Pharmacology and Clinical Therapeutics | Review and cite CLINICAL PHARMACOLOGY protocol, troubleshooting and other methodology information | Contact experts in CLINICAL PHARMACOLOGY to get answers

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Questions related to Clinical Pharmacology

What is role of the magnesium acetate in cell lysate?

I'm working on preparation of protein lysates from E.coli I read in a few paper that magnesium acetate should be added before sonication what is role of the Mg ace in cell lysis?

04 November 2023 4,830 2 View

Can nootropic be the perfect candidate for the pharmacotherapy of dementia?

Nootropic or commonly known as Smart drugs that acts through synaptic plasticity.

03 November 2023 3,224 0 View

Amperometry HCT correction for glucose measurement, how to find the HCT index?

Dear all, I am trying to find the HCT index for pursual of amperometry correction of glucose. I couldn't find much of the references for this. Can someone help me to provide more insight on this...

27 October 2023 9,664 0 View

How do I dilute some fungal extracts in DMSO and produce test concentrations with 0.1% final DMSO?

I have some fungus extracts to test against tumor cells (B16, K562, Kasumi-1, KG1), the only information I have about them is the amount in mg (4.37mg). How do I dilute them in DMSO? and how do I...

05 September 2023 8,778 2 View

Could you please guide me on how to apply equation to calculate the minimum, acute, and maximum values from the cell toxicity study data?

I wanted to use the conversion equation for human dose to animal dose: (ng/kg) = HED (mg/kg) / Km, for my experimental data from a cell toxicity study. I am interested in finding the minimum,...

04 August 2023 8,590 0 View

I want to know the sample preparation methods for LC-MS/MS using blood sample. Can we convert liquid sample into solid or semisolid form?

To check the metabolites of blood , I want to use LC-MS/MS. So I want to know more about the sample preparation and is there any method to convert liquid sample into solid or semi solid form.

29 June 2023 7,069 3 View

Synthesis of NaCMC?

We have synthesized carboxy methyl cellulose by 30-40% NaOH and after that adding chloroacetic acid (1.5 times) for 3 hours. The next day, methanol added and neutralized by glacial acetic acid and...

25 May 2023 5,245 0 View

How can sustainable land management practices be used to improve soil health, increase biodiversity, and reduce greenhouse gas emissions?

30 April 2023 6,382 6 View

What should be the adequate range of cry1Ac protein (ug/g) to kill helicoverpa armigera in bt cotton?

The protein dry wet expression range for leaves/square/bolls parts will be approximately similar or will be different? Is their any standard range?

21 April 2023 1,553 0 View

How do you calculate a method detection limit for rates in oxygen consumption?

We have collection oxygen consumption measurements using PreSens spot sensors in the PreSens Sensor Dish system and ran a 12 Milli-Q blanks. In this system you can continuously monitor the oxygen...

06 April 2023 9,704 0 View

Hi i want equations for calcium and magnesium calculation in water ?

I have the volume of edta but i need how to calculate calcium and magnesium by mg/l

05 April 2023 4,955 1 View

What is the role of steroids in acute epiglottitis in elderly adults with mild respiratory distress ?

Is there any role for corticosteroids in the setting of management of epiglottitis in elderly adults with mild respiratory distress ?

31 March 2023 5,648 0 View

Should I remove remaining materials in defect site before doing microCT scan and analysis?

Dear colleagues I plan to do microCT scan and analysis of 4 mm cranial defect model in rats. However, I am confused about several technical things. I did the bone defect treatment by using several...

13 February 2023 3,108 2 View

What is better to use to monitor CAF activation? Adult fibroblasts or fetal fibroblasts?

Hi, I have been having difficulties in monitoring CAF activation because the lung fibroblasts Wi-38 that I am using seem to already have high levels of expression of a-SMA, FAP and PDGFRb,...

06 February 2023 6,890 2 View

Has anyone had experience adding nortriptyline to a MAOI? Is it safe? Does it work?

https://www.psychotropical.com/attenuation-maoi-pressor-response-nris/

31 January 2023 5,781 5 View

I am searching for a commercially available RWPE-1 cell line to use as a healthy tissue control for prostate cancer research experiments?

Hello PCa research community, I am searching for an appropriate prostate epithelial cell line to use as a non-cancerous, non-tumorigenic, control for my PCa experiments. I came across the RWPE-1...

22 January 2023 4,634 1 View

Can Halo's disappear?

Hello, For school I'm doing a project on bacteria and plastic degradation. For the method I had a R2A agar plate, then I made a suspension of a bacteria and used a sterile cotton swab to put 5...

13 January 2023 3,413 3 View

Is 6xHis tag compatible with therapeutic (GMP) protein production?

Hello! We are producing a recombinant protein (from mammal cells) and we need a 6xHis tag for the purification. This protein is aim to be used in preclinical experiments in the short-medium term,...

12 January 2023 524 2 View

What is the simplest method for hematocrit correction during glucose measurement in electrochemical dry strip technology?

Hematocrit correction during glucose measurement is very important for enhancing the accuracy of measurement in whole blood. As far my knowledge, it done electrochemically either through impedance...

21 December 2022 8,485 0 View

What should we do to resolve the problem of formation of metabolites and disintegration of drug during drug release application of hydrogel ?

We are working on polysaccharide based hydrogel (containing high vitamin C), on its drug (Paracetamol also we used Methotrexate salt) release studies using phosphate buffer as a solvent it is...

08 December 2022 7,153 2 View

How to prevent spreading depression in mouse visual cortex slices?

I'm looking for tips on how to prevent spreading depression that has been repeatedly occurring in my mouse visual cortex slices. I've been washing out Mg2+ to induce low frequency oscillations in...

10 November 2022 6,800 0 View

What might be the reason Pseudomonas pyocyanin production not evident on nutrient agar?

Pseudomonas aeruginosa is not showing pigmentation when incubated at 37 degree centrigrade for 48 hours on nutrient agar plate. Is any component of the media responsible for it?

25 October 2022 9,755 0 View

Best statistical way to determine vitamin deficiency?

Hello Guys I have collected 9000 vitamin D samples and I want to know how to determine how many of the samples (having Vitamin D deficiency, Insufficiency, Normal, or toxicity). what I mean is how...

17 October 2022 9,676 1 View

What is the purpose of transplanting leukemic cells into healthy mice?

During my research about AML I read a lot about transplanting leukemic cells from mutated mice into healthy mice to see if they develope leukemic phenotype or not. What does it say about the...

11 October 2022 626 0 View

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