During my research about AML I read a lot about transplanting leukemic cells from mutated mice into healthy mice to see if they develope leukemic phenotype or not. What does it say about the leumeic cells if they develop a leukemic phenotype or not?
I am looking thrombospondin-1 protein in mouse cerebellum tissue. I can get band, but always there are dirtiness on the membrane. I tried different concentration of thrombospondin and also I used...
08 July 2020 3,321 3 View
I would like to learn is there any software for detecting Lymantria dispar nucleopolyhedrovirus hrs regions? I am working on a full genome sequence data and I need to search hrs regions in my...
05 July 2017 3,752 2 View
I am using Roche 3'/5' 2nd generation RACE kit to determine 3' and 5' utr regions. I have completed all steps and sequenced my 3' plasmids (pGEM-T Easy vector). The main point: my gene has a...
09 June 2014 2,499 1 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
01 March 2021 6,953 1 View
Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...
01 March 2021 9,938 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View
Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...
24 February 2021 1,825 3 View
Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...
24 February 2021 5,759 3 View
Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...
23 February 2021 7,701 2 View
Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...
23 February 2021 5,476 4 View
Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha
22 February 2021 3,891 2 View