During my research about AML I read a lot about transplanting leukemic cells from mutated mice into healthy mice to see if they develope leukemic phenotype or not. What does it say about the leumeic cells if they develop a leukemic phenotype or not?
Hello everybody! The amidation of 4-dodecyloxybenzoic acid methyl ester doesn't work at all according to the publication "Perylene liquid crystals with multiple alkyl chains: investigation of the...
04 June 2023 8,026 7 View
Dear colleagues, I would like to know how to determine the amount of functional groups, here carboxyl groups, in PAA (100 kDa). I would guess by acid-base titration. In detail I would prepare a...
08 February 2023 6,803 11 View
Hello everybody! I've modified a purchased polymer, here PMAA (100 kDa), with Dodecylamin in the presence of Dicyclohexylcarbodiimide and would like to precipitate it, which doesn't work...
02 February 2023 817 1 View
Good day to all, We are extracting and cultivating cortical neurons from P0-P2 rat pups. But they seem to stay depolarized in culture and we don't understand why. Morhologically they look good,...
03 February 2022 5,549 5 View
The correlation values between the sub-dimensions of two variables with different sub-dimensions are evident. However, the correlation value between the two main variables is not clear. How can I...
30 October 2021 9,013 1 View
Atrial fibrillation (af) ablation and cardiac mri What do you think about the evaluation of ablation lesions with postablation cardiac MRI, especially in patients with permenant atrial...
02 September 2021 5,178 9 View
I am looking thrombospondin-1 protein in mouse cerebellum tissue. I can get band, but always there are dirtiness on the membrane. I tried different concentration of thrombospondin and also I used...
08 July 2020 3,456 3 View
I am planning to edit a viral (insect virus) genome using CRISPR CAS-9 system. I need to find a suitable vector system for producing cas-9 and gRNA's in insect cell culture. Any suggestions?
27 November 2017 9,790 1 View
I would like to learn is there any software for detecting Lymantria dispar nucleopolyhedrovirus hrs regions? I am working on a full genome sequence data and I need to search hrs regions in my...
05 July 2017 3,965 2 View
GDP is not a perfect measure of well-being, and development has other aspects beyond economic growth. This is why there are other approaches to the welfare of an economy such as the Human...
25 January 2017 8,607 14 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View