Dear colleagues

I plan to do microCT scan and analysis of 4 mm cranial defect model in rats. However, I am confused about several technical things.

I did the bone defect treatment by using several types of hydroxyapatites covered with a collagen membrane in different animal groups. At the end of the treatments, some remaining materials and the whole collagen membrane are still left in the defect site. I am afraid that the remaining material will appear in the CT scan, making the bone growth analysis difficult. Because of this, I wonder if is it appropriate to remove the remaining material and the attached collagen membrane. My planned analysis is BV/TV and BMD.

If yes, I would be glad if you could suggest a procedure to remove them at the defect site considering that the cranial bone of the rat is thick and may easily be brittle.

Thank you very much.

Best regards,

Maria

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