Hello everyone!

I have an urgent question.

I have observed mitochondrial-genes downregulation (RNA-seq). I performed Mito-spy staining on cultured Schwann cells from wild type and can see proper staining and conc. used was 400 nano molars.

Now I will be performing Mito-staining for transgenic Schwann cells. I was wondering whether dye conc. 400 nano molar is a saturated concentration and may be due to this I won't be able to differentiate between KO and the wild types.

As I am doing this Mito-staining first time and interested to know what point I should consider optimizing the condition so that can clearly differentiate between two groups.

suggestion will be appreciated.

Thanks in advance