Pseudomonas aeruginosa is not showing pigmentation when incubated at 37 degree centrigrade for 48 hours on nutrient agar plate. Is any component of the media responsible for it?
I am trying for the structure optimization via Gaussian16 DFT calculation, for a system consisting of C,H, N, O and Ce atoms. Where I am using B3LYP/ 6,311G(d,p) C, H, N and O and B3LYP/SDD for...
30 June 2024 9,979 4 View
What basis set/basis sets will be useful for DFT (using Gaussian16) of cerium ion adsorption?
24 June 2024 6,927 3 View
Suppose I need to target mitochondria to address malignancy-related problems. Are there peptide-based ligands similar to Nuclear Localization Signals (NLS) for nuclear targeting that can be used...
20 June 2024 9,587 1 View
Kindly mention the protocol
27 May 2024 3,243 0 View
What are the force field parameters for TIP4P-Ew water model? Including the force constant of Oxygen-dummy atom bond length, and hydrogen-oxygen-dummy atom angle? So, basically, what is the...
08 April 2024 3,426 0 View
kindly provide some insights
01 April 2024 4,674 2 View
To culture the U-87-MG cell line, various media formulations are available online. These include: a. Essential Minimum Eagle Medium (EMEM) supplemented with 10% fetal bovine serum (FBS), 100 U/mL...
07 January 2024 612 0 View
For the past few weeks, I've been attempting to passage U-87-MG cells in our lab. However, after thawing, instead of reaching 80% confluency within 24 hours, they're taking 3-4 days to achieve the...
21 December 2023 509 2 View
I have used Trizol method but there has been huge DNA contamination along with shearing.
17 December 2023 4,745 4 View
I understand that ED-XRF or WD-XRF only allows for high energy photon emission. I am wondering if it is possible to obtain emission in the visible region by attaching an external optical fiber to...
29 November 2023 2,939 3 View
Pig slurry is rich in major and minor nutrients. Is there any way to improve / Enrich its manure quality to be used in agriculture organically ? please share your knowledge.
09 August 2024 5,605 2 View
I am working on microalgae cultivation using waste water. The initial concentration of nutrients were less but the microalgae has achieved biomass growth of 2 g/L. The final concentration of...
08 August 2024 4,812 2 View
How is matter moved through an ecosystem and movement of energy different from the movement of nutrients through ecosystems?
03 August 2024 4,859 0 View
Why does the flow of energy through the biosphere depend on the cycling of nutrients and matter and energy flow across the earth's subsystem?
03 August 2024 6,412 0 View
How is the flow of matter through an ecosystem different from the flow of energy matter and nutrients flow through an ecosystem?
03 August 2024 8,655 0 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
One has to list down the details and understand that different feedstocks and the biogas digesters working on different feedstocks show different nutrient content value: But when it comes to...
18 July 2024 9,907 12 View
I am working on probiotic bacteria for fish, and I am trying to isolate probiotic bacteria like lactic acid bacteria or other probiotics from the gastrointestinal tract of fish using different...
12 July 2024 3,756 4 View
In the modified carbapenem inactivation method (mCIM) and EDTA-carbapenem inactivation method (eCIM) protocols, can we replace blood agar with another culture medium?
10 July 2024 6,352 0 View