Hi,
I'm currently running DNA (300 bp) on 8% acrylamide gels for heteroduplex analysis. I loaded samples 2 times on the same gel (I loaded the first samples and let it run for 4 hrs and then I loaded another set of samples and let it run over night at 200V), but I've seen a strange band/migration on the gel and I don't what's wrong with it or how this happened. Please have a look at the picture (https://ki.box.com/s/0rzvhjbwmws02b9jwadkfj4ew4uo28ld)
Also, take in to consideration that I have repeated the same experiment now (with new TAE buffer, checking that the buffer covers all wells and that the buffer is cold) and I get the same band on the gel.
Does anyone know what causes this problem?
Appreciate any suggestions!
Thanks
Ghazaleh