04 April 2015 1 9K Report

Imbert and Cullander already published a method in 1999 to investigate tissue viability by MTT assay. The authors said tissue samples were incubated in 2 mL of 2-mg/mL MTT (dissolved in PBS) for 2 h at 37°C (250 rpm). After incubation, the remaining MTT was removed, tissues were rinsed twice in PBS and then minced with surgical scissors, and formazan precipitate was extracted in 4 mL of DMSO at 80 rpm for 80 min. My questions are: could I homogenize my tissues in DMSO instead of mincing them? If so, after homogenization, should I centrifuge samples to collect the supernatant? Would the supernatant contain the soluble formazan in DMSO? If you have any other considerations or references, please send me.

Similar questions and discussions