Does anyone know a protocol for isolating epithelial cells from skin or buccal mucosa biopsies shorter than the 16 hour protocol with Dispase?

04 April 2015 0 7K Report

I'm trying to isolate oral epithelial cells, and currently using a modified protocol outlined by Izumi, Marcelo and Feinberg ( 18 hour incubation with Dispase followed by 2.5 hours with Collagenase IV) However, I would like to use a shorter protocol that takes lesser time, and perhaps only one incubation. Does anyone have any ideas?

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