I make it at 1mg/ml in sterile PBS. I start with sterile powder as I am working aseptically. It forms a suspension so it will stay cloudy and it shouldn't be filtered. Just make sure to resuspend well at every step to assure uniform dispersion. I freeze it down in small aliquots for single use in my assays. Never had any issues.
You can dissolve Con A in PBS or in RPMI-1640 at concentration 100 mkg/ml. You can filter solution via standard 0,2 mkm pore size filters for sterilization. You do not need to freeze Con A solution. Sterile solution is very stable at 4(o)C in tightly closed flasks in simple refrigerator. Final concentration of Con A in mouse lymphocyte cultures should be 3-5 mkg/ml. So, you should add 6-10 mkl of Con A solution per well of 96-well plates.
NO!!! I use micrograms (1 mkg = 10(-6) g.) This rank of concentrations seems to me more comfortable than one that uses Molly.
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