What is the best way for quantification of a FlAG-tagged protein?

Anti-FLAG ELISA can be used but the problem is that we do not have an antibody against our protein. Any suggestion will be appreciated. Thank you

11 January 2021 8,458 1 View

Could Anyone suggest the protocol for staining CD 4 and CD 8 in whole blood. Which buffer is suitable for the staining?

I am staining the CD4 and CD8 markers in the whole blood sample. However, when I used the PBS buffer (pH. 7.0 to 7.4) I have no results. Then I tried 2mmEDTA PBS buffer but still, there is no...

24 March 2020 252 3 View

Where can I buy vaccinia immunoglobulin (VIG)?

Hi, I need to perform a neutralization assay for an orthopoxvirus. I thought VIG should be easily available to purchase. However, I could not find a company to purchase it from. Most people seem...

04 February 2020 6,369 2 View

How to proceed for the counting of CD4, CD8 and CD19 from whole blood sample without isolating the WBC?

I am doing experiment with BD antibody conjugated with CD4 FITC, CD8 PE and CD19 perCp. However, I am not able to detect with higher concentration also. I need some information regarding the...

23 October 2017 8,042 2 View

How to count the live and dead cell and how to distinguish except the trypan blue technique. As, I know trypan blue only stains the dead cell?

05 February 2017 2,591 3 View

What solvent (and concentration) is suitable to dissolve essential oil (water or DMSO)? Is there any complication for using it for cell test?

30 September 2015 9,883 13 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View