Hey, I do have a question with murine GM-CSF and M-CSF for making rat BMDCs and BMDMs? I want to use WEHI 3B and L929 conditioned cell culture media in order to generate rat BMDCs and BMDMs.
I've already cultured BMDMs. You can indeed use L292 media; these cells are good producers of M-CSF. Final concentration should be around 30 ng/mL so the volume of the L929 media will depend on the concentration of M-CSF.
I also use recombinant M-CSF and I can tell you that both are very efficient.