I am making hypoxia situation in human pigment epithelial (RPE)using CoCl2.
I read the some paper that the cobalt could make hypoxia situation and reduce the cell viability.
however i used from 50 to 500uM in the serum free medium for 24h,
the viability is increased. The viability is measured by CCK-9
What do I wrong?
My RPE is passage #7 and cultured in DMEM with 10% FBS.