I have checked the peroxidase activity of E.coli under the stress of silver nitrate (20 μg/ ml). Surprisingly, I am getting negative results means control sample (E.coli without any silver nitrate treatment) found to have higher peroxidase activity silver then treated bacteria. Can anyone suggest what could be the probable reason behind this kind of result ?
Please provide me the reference if you can.
Can you please explain what you intend to say and If possible please share any research article which states the same, though I can cite that particular article .
Since silver ion is toxic to bacteria, if the treatment was too strong you may have killed off a large portion of the bacteria. With fewer live bacteria in the treated group, there would be less peroxidase activity.
Does silver ion inhibit peroxidase enzyme activity? If so, did you remove the silver ion before testing for peroxidase?
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