What are the substitutes to Sodium azide and EMS as cytotoxic agents?
N-ethyl-N-nitrosourea, Colchicine and UV/Gamma rays are the alternatives I know off.
my gene of interest size is about 4kb. I used hi fidelity taq pol as well as Q5 from NEB but still its happening. my forward primer composed of a 4 adenosine then restriction site followed by 6x...
05 May 2024 4,558 2 View
The band gap of metal-semiconductor-based core-shell nanocomposites is very narrow compared to their semiconductor nanoparticles. Is it characteristic of core-shell structure or any other doping...
31 January 2024 8,512 4 View
All the files are placed in a specific folder in C drive. (it includes the protein and ligand pdbqt files, autodock4.exe, autogrid.exe and dat files from mgl tools 1.5.7). After repeatedly running...
25 December 2023 5,761 3 View
I am dealing with a company profile that was established in 2005. For most of the variables, the information is available either till 2021 or 2018. My concern is whether a time series ARDL...
05 November 2023 2,897 2 View
Relation to determine trap energy from J-V characteristics obtained at varying temperatures of solar cells and expound upon the optoelectronic parameters based on the trap energy.
30 August 2023 9,899 2 View
I have understood the theoretical concepts of moderating and mediating variables. However, the research papers that have tested the roles of such variables are using interaction terms for both...
03 July 2023 2,442 4 View
Most of the research papers who have conducted the Autoregressive Distributed Lag (ARDL) regression method and are published in top quality journals do not show the multicollinearity tests in...
27 June 2023 7,145 0 View
Requested scientific theories and comprehensive experimental data.
13 June 2023 703 3 View
We have the cryopreserved form of Plasmodium beghei ANKA stain in liquid nitrogen vapour in our lab. We are for the first time going to inoculate the mice with the stain to induce rodent malaria...
17 May 2023 3,451 0 View
I am performing the Breusch-Pegan test and it is showing an insignificant p-value. Also, the Hausman test also shows an insignificant p-value. I wanted to ask if OLS, Fixed-effect or...
01 March 2023 8,001 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
What is the cycling of matter through ecosystems and how energy drives the cycling of matter in photosynthesis and cellular respiration?
02 August 2024 9,518 2 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...
28 July 2024 2,979 1 View
PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...
28 July 2024 4,762 2 View
Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.
28 July 2024 6,501 0 View
Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
25 July 2024 4,927 3 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View