Does anyone have a pGWB435 vector or any other LCU vectors with the R4L1 site?
If you have any information, please give me an email address. or send me an email to [email protected] you very much!!!
Hello, In the standard TMT SPS MS3 method, ion trap is used as detector for ms2 data acquisition. In my experiment, it would be much beneficial to use Orbitrap for ms2 data acquisition to obtain...
26 May 2024 628 0 View
Hi all, I have been trying to knock out genes in Yarrowia lipolytica using CRISPRyl system, but it always appears right for initial verification by colony PCR (PCR products shorten as up/down...
17 January 2024 5,089 0 View
Hi! I have questions to consult with scholars. We consider a multiuser MISO downlink communication system where an IRS equipped with a UPA ( with M reflecting elements) is deployed to enhance the...
08 December 2023 6,883 4 View
Hi! I have questions to consult with scholars. We consider a multiuser MISO downlink communication system where an IRS equipped with a UPA ( with M reflecting elements) is deployed to enhance...
07 December 2023 7,563 2 View
I want the "Sn" to hang on the end of the polymer,but experiments have been failing lately,do you have any suggestions?
29 November 2023 3,905 1 View
Thanks for any help, this is my first time conducting DNA experiment. I want to hybridize a 26-base-DNA to 38-base-DNA and then use a 100-base-DNA to replace the 26-base-DNA, is this promising to...
28 September 2023 5,047 0 View
Regarding in vitro electrophysiology, why do I see that some literature evaluates the intrinsic excitability of neurons with APV, CNQX, Bicuculine in the external fluid(ACSF), and some are...
27 September 2023 8,099 3 View
first time asking question, trying to use UV light to excite Sn and SnO2 and detect the fluorescence, however there is nothing be detected. help
08 August 2023 4,537 4 View
In order to better study the glass-crystal transition of specific inclusions during heat treatment.
10 July 2023 577 0 View
Hi! Recently, I found an interesting article, titled "Abeywickrama, S., Zhang, R., Wu, Q., & Yuen, C. (2020). Intelligent Reflecting Surface: Practical Phase Shift Model and Beamforming...
19 June 2023 4,542 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?
15 July 2024 1,598 3 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...
10 July 2024 6,830 1 View