How to calculate degree of deacetylation of chitosan? Please guide in this regards
I am learning microstructure modeling and also looking for some resources and opensource software to start with.
05 April 2024 4,576 3 View
Is it correct to treat RAW264.7 mice macrophage cell line with recombinant Human Macrophage Migration Inhibitory Factor (MIF). Will there be any species incompatibility related unwanted effect on...
12 November 2023 1,534 1 View
Hi I was wondering whether CMV and EF-1alpha promoters can be put in a single plasmid? Are there any compatibility issues between the two promoters? Thank you.
31 October 2023 9,919 2 View
I am looking for the ED50 of meropenem in mice against Klebsiella systemic/lung infection models. Is anybody aware of it? Thank you.
18 June 2023 2,018 0 View
I want to know which software is used by authors to draw schematic diagrams of beams, plates etc. for there articles.
22 May 2023 7,631 6 View
Hi I am kind of looking to see if a comparative expression level analysis of the different Glycosylation enzymes of different CHO cell lines (DG44, DXB-11 and K1)is available in any publication. I...
12 April 2023 9,492 2 View
Most of the available literature related to vibration of nanobeam with elastic support boundary condition, author use Differential Transform Method (DTM) to solve the governing differential...
19 March 2023 3,763 2 View
I am trying to replace a 20 bp region with a different sequence of same lengthin a 10kb plasmid. I designed overlapping primers. However after PCR I am only getting the template. At first I...
13 February 2023 3,663 3 View
During conformational analysis of 18crown6, it was observed that the conformer with S6 symmetry was having the lowest energy when dispersion correction was not included. However, upon addition of...
14 February 2022 1,493 3 View
I am looking to study opsonophagocytosis using THP-1 cell line from ATCC. Does the cell line require differentiation before the phagocytosis assay?
28 October 2021 3,412 5 View
My final research is about making chitosan/pva hydrogel for wound healing using gelatin as crosslinker. Last month, i made the thin film hydrogel and it turn well. It was okay. Last week, i make...
28 July 2024 2,794 6 View
I am trying to load curcumin emulsion in 4% chitosan gel but i don not know how many mg powder is needed ?
10 July 2024 2,462 1 View
Hi, I am synthesising chitosan nanoparticles using the ionic gelation method and I am characterising the nanoparticles in distilled water and in DMEM (cell culture), however the zeta potential in...
01 July 2024 1,526 1 View
Hello, I want to load Remedesivir (RNA polymerase inhibitor) into mesoporous nanoparticles (MSNs). The issue is drug is temperature and pH sensitive and it degrades quickly. I need advice on its...
01 July 2024 889 3 View
I am currently trying to measure the entrapment efficiency of curcumin in liposomes. Typically, this measurement can be done by centrifugation, and I have followed the protocol outlined by Asif et...
01 July 2024 7,007 1 View
I need to magnetic stirrer chitosan for 24 hours. In this case, if I magnetic stirrer the chitosan solution for 6 hours, then I take 10 hours of breaks. I followed this 4 times so that the overall...
31 May 2024 9,202 0 View
Hi everyone, there is my issue: I try to synthetize nanoparticule of Chitosan according with this study doi: 10.1016/j.colsurfb.2011.09.042 I try it with HMW Chitosan (340-360kDa) but when I put...
29 May 2024 1,763 2 View
A uniform mixture of chitosan and gelatin is made by dissolving in acetic acid, after that the mixture is transferred to a plastic petri plate kept at -20 C for 24h and then lyophilized. But i am...
29 May 2024 7,367 4 View
I have prepared some polymeric microspheres from chitosan and PCL. However while performing in vitro degradation study, I found difficulties in both above mentioned methods. Here I am mentioning...
20 May 2024 6,114 4 View
We prepared a solution using silver nitrate, chitosan, and sodium tripolyphosphate to synthesize silver chitosan nanoparticles. This solution was subjected to cold atmospheric pressure plasma for...
19 May 2024 9,317 2 View