For lipid extraction, I am using 2/1 CHCl3/MeOH added to the cell suspension followed by centrifugation. This results in 3-layer separation. But while taking bottom organic layer, middle protein layer is also coming as it is very fragile. In the next step, I am adding formic acid for acidic lipid extraction. Will it help to add 1M Nacl to improve bottom layer extraction? Also I am adding 5% formic acid during extraction. Is it too much?

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