Dilute and shoot for free fatty acid analysis?

05 June 2023 1 2K Report

Hi everyone,

I am looking to develop a method for analysis of free fatty acids in oil (coconut, canola, olive... etc.). I've noticed that there are some methods out there that simply use isopropanol to dilute the oil and then they do MS scan (usually high resolution) to profile the acids. Does anyone have experience with LC/MS analysis of fatty acid content of oils? Seems a bit to simple to just dilute-and-shoot oil into an instrument (sounds like a dirty sample)... but I could be wrong! Can you please share your thoughts with me? Thanks!

Zainal Kifli

If you want to determine the profile of the free fatty acids (FFA), then you have to separate the FFA first from the rest of the oil. Then you have to convert it to FAME before injecting it into a GC. But if you want to determine the fatty acid profile of the whole oil, then just simply convert the oil into FAME.

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