I want to do SEM analysis for Alginate hydrogel samples, how long do I need to lyophilisation to freeze drying the hydrogel?

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Budharaju Harshavardhan

We usually do it for 36 hours. For 1 mL of alginate hydrogel, 24-36h is sufficient.

Along with the volume of hydrogel, you should also consider the concentration of alginate.

Betül Mutlu

I think a continuous freeze-drying process of 24 hours would be sufficient to lyophilization 1 ml of alginate hydrogel.