I am using propidium iodide (Hi-media Product) but i want to know how much Concentration of propidium iodide is required to stain in 100 cells ?
Hi..You can't prepare a PI solution that exactly stains 100 cells....Normally PI stock solution is supplied @ concentration of 1 mg/ml .. Dilute it to 1000 times in water or PBS and use it for staining the cells.....
hmmm.... ok. Thank you Ashwin.
11 January 2021 3,284 3 View
I need to process an image for edge detection and then want to count the number of edges in that image.
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I am searching to find commercial vendors who sell SWIR dyes. I could only find Nirmidas Biotech which sells different NIR-II dyes!
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doi of your presentation in the conference
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We working on PCL nano particles 1).Ketoprofen (10mg)+ dmso(5ml) Pcl (10mg)+ DCM(5ml) 2).Water 10 ml+ 10 mg When we mix both 1+2 after solublization (400 rpm) Why precipitation of polymer occurs?
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I want to establish DOX/R cell line in vitro for the research study.If any one suggest the protocol then it would be a great help.
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What is the difference between weak from Ritz method and variational form Ritz method while solving a boundary value problem ? Can all differential equations take variational form ?
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Hi, We are working on DAR (Drug to Antibody Ratio) estimation of ADC using the following HIC-UV method. For some of the unconjugated mab we getting a sharp peak but for our desired mab we are...
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I have attached an excel file with the quantification of a mineral I conducted EDS on. I have more excel files of minerals too as I know EDS spectra for only 1 sample will certainly not be...
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I performed a boc deprotection via 25% TFA/DCM (1 hr) without any workup, which should yield TFA salt as a product. My compound also had a TBDMS group on it. But when I took an NMR, the amine peak...
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Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...
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Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...
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Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?
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how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...
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Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
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Please provide Book Title and author name
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An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...
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