I am using propidium iodide (Hi-media Product) but i want to know how much Concentration of propidium iodide is required to stain in 100 cells ?

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Is Vacation Homes and Vehicle Rentals in India succesful?

Assessing the Feasibility of Vacation Homes and Vehicle Rentals in India In my article, "Assessing the Feasibility of Vacation Homes and Vehicle Rentals in India: Understanding Market Needs,...

03 July 2024 5,535 1 View

How the electron and hole pocket near the fermi level in the band structure affect thermoelectric properties of Topological half-Heusler compound?

How the electron and hole pockets near the fermi level in the band structure affect the thermoelectric properties of the topological half-Heusler compound?

26 June 2024 2,933 0 View

What is the difference between uv visible measurment and phocatalytic degradation measurment?

why uv is done and why photocatalytic measurement is done although both use uv radiation

09 June 2024 8,750 1 View

By mistake my friend dipped Ag/AgCl reference electrode in KI instead of KCl now is is not working then what should we do to save the electrode?

this electrode can't be open.

13 May 2024 2,991 3 View

Can i use ASTAM D 1002 for lap shear strength of wood substrate ?

which ASTM D USE for lap shear strength in UTM of wood substrate

04 April 2024 4,791 0 View

Where did i got my research gate id?

21 March 2024 5,116 0 View

Anyone suggest me other lysotracker dye?

i am doing Colocalization Studies i used Hochest 33342, cell mask and Lysotracker deep red, orange and red. please any one suggest me another lysotracker dye and lysosensore dye according this...

21 February 2024 8,279 0 View

How to change a Dihedral angle of lipids molecule in Gromacs/Charmm gui ?

Dear all I'm trying to prepare a lipid bilayer system having more than 100+ no of a specific single lipid. I want to change the conformation of cis bond of lipid into trans form and then perform...

04 February 2024 6,409 0 View

How to prepare a lipid bilayer membrane having trans conformation for MD simulation?

Hello everyone Can anyone please suggest me how I can prepare lipid bilayer molecule having trans conformation of double bond of lipids when the trans conformation of that specific lipid (ex...

04 February 2024 3,205 0 View

I have an EPDM rubber that has swelled in oil. What solvents can bring back the rubber to the original shape?

24 January 2024 7,796 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

May members post flyers about opportunities to present at a conference? If so, where to post?

May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/

08 August 2024 4,585 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Hello all, Looking for international reviewer to review Ph.D thesis in wireless sensor network.Can anybody help?

My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...

07 August 2024 4,600 2 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View