I'm looking for a marker specific to T helper 3 cells (Th3) for use in a multicolor flow cytometry panel. Any suggestions for a specific marker, or combination of markers, is greatly appreciated.
I have not found or know any specific markers for Th3 cells.
However they should secrete TGF-b, so if you want to characterize them with flow cytometry one could block secretion of cytokines with a GolgiStop and do an intracellular fowcytometry for TGF along with other surface markers like CTLA-4 and CD25.
You could also refer to this paper. It can guide you to differentiate Tregs from Th3 cells