What is a good marker for Th3 cells in multicolor flow panels?

More Carl Manner's questions See All

How to illustrate FE Panel Regression with LSDV in a research framework?

First time to use FE Panel Data Regression using LSDV, and not familiar with how to illustrate it in a research framework. We have regional CSR score as IV and Social Development ( four areas:...

14 January 2021 580 3 View

Context in a Quantitative Context Analysis?

Hello, I am writing my bachelor thesis right now and am conducting a quantitative context analysis looking at how terrorism is portrayed in newspapers, but certain words could be used to describe...

04 May 2020 8,762 3 View

Species that contains the grass allergen Phl p5?

Does anyone know in which species the grass allergen Phl p5 occurs? It was originally isolated from the grass species Phleum pratense so it must at least occur in that species. It stand to reason...

15 March 2020 8,703 4 View

No title

24 February 2020 6,469 1 View

Does anyone know a freeware tool that can align exon and intron sequences to a template DNA sequence?

I am seaqrching for a tool that can align exon and intron sequences to a template DNA sequence without disrupting any of the short sequences. I remember that DNAStar was able to do this. Does...

12 December 2019 8,902 3 View

Direction and Wind speed using 3D Ultrasonic Anemometers?

What is the most accurate way of calculating wind direction (2D NESW), wind speed and vertical wind movement using 3D Ultrasonic Anemometer data in the form of uX-uY-uZ m/s (or U-V-W vector) data?...

22 October 2019 8,475 4 View

What is the link between a country's export performance and its national innovation international ranking and or reputation?

20 March 2019 8,010 4 View

Where can I find the following Italian agricultural text?

Hi, I am looking for an Italian agricultural text which seem to be difficult to find, can anyone provide me with a .pdf or the location of where I can acquire the text? Barbieri R., Botarelli L.,...

29 January 2019 3,317 2 View

No title

Hi there, I need a bit help in statistics. I developed an analytical method, which relies on calibration curves. For calibration, the measured signal value of a calibrator is plotted against...

03 November 2018 7,402 6 View

Can I Store Cells in -80 for later protein sampling?

Hello, I wonder is it possible to store the cells (Plate) at -80 degrees Celsius for later protein sampling (western blot)? I want your opinions

01 November 2018 2,819 6 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Mohanraj Ramachandran

Hi,

I have not found or know any specific markers for Th3 cells.

However they should secrete TGF-b, so if you want to characterize them with flow cytometry one could block secretion of cytokines with a GolgiStop and do an intracellular fowcytometry for TGF along with other surface markers like CTLA-4 and CD25.

You could also refer to this paper. It can guide you to differentiate Tregs from Th3 cells

http://www.jimmunol.org/content/171/12/6323.full.pdf