I am working with exosomes purified from whole saliva samples. I would like to know the best RNA kit to isolate both mRNA and miRNA from exosomes for microarray profiling.
I appreciate any response.
Hi Marcos! We use HBM Overall Exosome Immunocapture and RNA extraction kit http://exotest.eu/online_orders/rna-kit/overallRNA
Good luck!
MiRvana kit is what we use to isolate miRNA from exosomes. It will also isolate mRNA. We had originally just used trizol isolation, but found we lose most of the miRNA.
Thank you guys. I'm afraid I will have to use two kits as I need to isolate both mRNA and miRNa but your answers were really helpful.
Thanks a million
no problem, I'm almost positive that mirvana has two protocols and and of them isolates total RNA while the other further enriches for miRNA. You might be ok with just the one kit in that case.
That is fine. I have just read about a TermoFisher kit that isolates both RNA and protein from exosomes. Not sure if it is reliable though as I did not find anyone using it.
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