I am targeting bcl2 and bax pathway in breast cancer cells, MCF7 and MDAMb231 and my drug may block this pathway. According to my hypothesis, I will use western blot and qRT-PCR to confirm this effect. Should I use a positive control to study its effect in this pathway? if yes, which positive control I should select?
Prafulla Chandra Tiwari
Dear Siddarth this could b helpful to u http://www.nature.com/leu/journal/v28/n8/full/leu201444a.html
0 votes
0 thanks
Vadim Markovtsov
ABT-737, ABT-199, ABT-263, AT101, GX15-070 are all good controls, especially ABT ones. Available from multiple vendors, for example here:
http://www.selleckchem.com/products/abt-199.html
0 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Medicine
- Oncology
- Cancer Research
Similar questions and discussions