SiHa, HCT116, HT-29, and FaDu cell lines have been widely used in tumour hypoxia studies. Any particular reason(s) for selecting those lines? Thanks everyone :)
The major reason is the hypoxic conditions in the tumor micro-environment in which each cancer cell line was established. For examaple, colon cancer expresses and secretes large amount of VEGF, but tumor cells tend to be exposed to hypoxia considering the aberrant proliferation. Besides, HIF1-alpha induction is also easily to occur in those cells in the experiments using CoCl2 mimicking hypoxia.
I agree with the above answer, I'd also say that in xenografts (like for FaDu: http://altogenlabs.com/xenograft-models/other-bladder-cervical/fadu-xenograft-model/) you get very consistent growth patterns that can be linked to hypoxic conditions and drugs that affect hypoxic pathways (HIF-1 targeting, for example). In general, these cell lines are well characterized, and there is lots of literature giving reference data for hypoxic conditions and their effects on the cells, which helps a lot when making a hypothesis.
- Badges
- Science topic
- Similar topics
- Medicine
- Oncology
- Cancer Research