I read some papers and most of them listed DO-7. Also, what other antibodies are better for p53 detection in terms of quality and reactivity and that are cost effective.
Before using / choosing a TP53 monoclonal antibodies, you have to define the purpose of your experiment, the method and the species of the target.
Both DO7 and DO1 are specific for primate TP53 and both bind specific epitopes localized in the amino-terminus of the full length protein. Therefore, if you want to detect the short TP53 isoforms that miss the amino-terminus of the protein, both DO7 and DO1 will miss these isoforms.
If you want to detect all TP53 isoforms, choose either a poly clonal antibody or a monoclonal that binds to the core region
If you want more info, let me know
I have tested multiple p53 antibodies and the best one was DO1 (I particularly like the one from Bio-rad as worked better than the abcam and CST for this protein. It can detect wt despite being a low expressing protein, the mutants, as those accumulate and also the truncated forms. some of the others fail to recognize some of those forms.
https://www.bio-rad-antibodies.com/human-p53-antibody-do-1-mca1701.html?f=purified
good luck
Ernesto
We have used p53-DO1 mouse mAb(EMD Millipore) and it recognizes FL as well as truncations very well.
Check this article for more information. Good luck
https://elifesciences.org/articles/17929
Before using / choosing a TP53 monoclonal antibodies, you have to define the purpose of your experiment, the method and the species of the target.
Both DO7 and DO1 are specific for primate TP53 and both bind specific epitopes localized in the amino-terminus of the full length protein. Therefore, if you want to detect the short TP53 isoforms that miss the amino-terminus of the protein, both DO7 and DO1 will miss these isoforms.
If you want to detect all TP53 isoforms, choose either a poly clonal antibody or a monoclonal that binds to the core region
If you want more info, let me know
Where can I get the information about the region recognized by each antibody?
Thanks
- Badges
- Science topic
- Similar topics
- Medicine
- Oncology
- Cancer Research