I'm looking for a protocol for doing fluoresence microscopy to see the mitochondria shape/AR, on PBMCs from human blood?

I'm looking for a protocol for doing fluoresence microscopy to see the mitochondria shape/AR, on PBMCs from human blood?

05 November 2020 8,841 3 View

Positive value in NCT real-time PCR?

Hi I will be happy for your help. I'm doing real-time PCR with two primers called MT-TL1 and 18S, for mtDNA and houskeeping gene, and I got positive control on both NCT. I tried also different...

07 March 2020 3,953 4 View

Hall effect: what is the electric conductivity tensor for AC magnetic field and DC electric field?

solid-state-physics I need to define electrical conductivity anisotropic tensor in a metal caused by Hall effect. My problem is that i don't know how to define it in case of oscillating magnetic...

19 January 2019 6,160 4 View

Cyclooctatraene damaged by TFA?

16 February 2021 4,747 3 View

Problem in detection of a specific gene & elimination of primer dimer in qpcr?

In our lab, we are trying to do real time PCR for some genes. One of them has a size of 85 bp. Whatever we do, we had an amplification in negative control. When we run agarose gel, it always a...

14 February 2021 9,750 11 View

Why non-specific band appear on T-ARMS PCR?

Hi everyone. We want to know how can I remove the non-specific band amplification on this variant. Although the amplification of non-specific band is not as intense as the band that we want, but...

09 February 2021 588 2 View

I have faced a problem to amplify VP2 gene of porcine parvovirus 1,2,3 and 5? Please suggest me how can i solve this problem?

Till now i haven't got any PCR amplified vp2 gene bands on specific annealing temperature and in step up PCR.

08 February 2021 819 4 View

Xrd result of ZnO thin film shown better crystallinity at low temperature and the crystallinity decreases as the temperature increased. why?

I have synthesized ZnO thin films at four different substrate temperature say, 300, 350, 400 and 450C. and the samples were annealed at 450C for half an hour. the xrd result shown better...

04 February 2021 7,641 4 View

How can I partially remove the metal oxide layer for solar cell device?

Hi, I want to make a perovskite-based planar solar cell device. (electrode/HTL/perovskite/ETL/FTO) To fabricate the device, one electrode should be deposited on HTL and the other should be...

01 February 2021 229 6 View

Are there any technical and safety tips or details for hot salt baths?

29 January 2021 6,036 2 View

Do you have experience of second round PCR amplification issue?

When 1st-round PCR amplification was finished , I could get a clear expected band, but the amount was very low, so I cut the band and run second-round PCR with same condition, it didn't work, I...

21 January 2021 888 4 View

Sb2Te3 thin film annealing and damage(break)?

21 January 2021 9,642 2 View

Strategies for short hairpin RNA interference cassettes?

In your experience, how much does the distance between the U6 promoter and the annealed -short hairpin - oligonucleotides affect the RNAi cassette expression? How to clone a shRNA sequence...

16 January 2021 9,418 2 View