I currently doing a research for anticancer drugs and have a plan to use CA 15-3 as tumor marker to measure the effectivity. Some reference tell that CA 15-3 and MUC 1 are synonym, but i also found some reference that stated CA 15-3 and MUC are different. My question is "Could I use ELISA kit for MUC 1 to measure the CA 15-3?" because my local supplier can't find the ELISA kit for CA 15-3 in affordable price.
Depending on what you want to adress you can look at cell proliferation via TK activity as a marker of efficacy. It has been proven superior to CA 15-3 in a study of locally advanced and metastatic breast cancer (Bjohle J et al BCRT 2013) and TK activity can be measured with an ELISA, DiviTum. Results from other studies of MBC and tumor types are also available
CA 15-3 is the circulating c-part of mucin 1 which was sheded by action of proteases. Therefore you have to use specific ELISA for CA-15-3.
Yes, you can use it in principle, but this also depends on what will you address. In general CA15-3 can be used as a marker, however, i would prefer to validate and confirm it via circulating tumor DNA.
yes. you can use it based on the principle. i used enzyme immunoassay for the quantitative determination of breast cancer antigen CA15-3 in human serum. I used it in a previous search.
Yes you can use ELISA for MUC1 if you don't avail CA15-3 ELISA for anticancer drugs assay
- Badges
- Science topic
- Similar topics
- Medicine
- Oncology
- Cancer Research