1- Sucrose 10% containing protease inhibitors 2-10 mm Tris-HCL, pH = 7.5 and supplemented with the ready-to-use 1× protease inhibitor cocktail 3-Tris buffer (pH = 7.4) containing 10 mM Tris, 10 mM MgCl2 and 1×EDTA-free protease inhibitor 4-75 mM sucrose, 20 mM Tris⋅HCl (pH = 7.5), 2 mM MgCl2, 10 mM KCl, and one tablet of protease/phosphatase inhibitors
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