A platform for people who work in the area of cancer biology | Contact experts in Cancer Biology to get answers
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Questions related to Cancer Biology
I am interested in finding transcription factors binding to promoter of a virus, Merkel cell polyomavirus. I got several factors with varying dissimilarity. Is there any criteria that can be used...
05 May 2016 753 2 View
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05 May 2016 3,222 3 View
We are planning to isolate glioblastoma cells for primary culture. After that we would like to establish a cell line from those primary cultures. I would like to ask the protocol for cell line...
05 May 2016 9,864 3 View
Has anyone tried the SUnSET assay with Puro-resistant cells? Is it possible? Could you share the protocol, if it needs adaption for that case,...
05 May 2016 4,950 1 View
I need MRI images of Glioblastoma multiforme cases, data for which is available on The Cancer Genome Atlas (TCGA). However, I am not sure if the portal contains MRI images available to...
05 May 2016 9,365 2 View
Tumor generated in mouse with chemical agents. Need to study above mentioned parameters after sacrificing the mouse. Would prefer a dye base flow cytometry assay rather than anibody based assya.
05 May 2016 7,366 4 View
How to product L929-conditioned medium for differentiate BMDM?
05 May 2016 5,296 2 View
Would you please suggest an in house protocol of single emulsion solvent evaporation to follow for encapsulating Artesunate into PLGA polymer?
04 May 2016 5,622 2 View
pathophysiology of carcinogenesis
14 April 2016 8,099 7 View
Hi All I would like to know if any one knows the knockdown cell line with lentivirus. I mean I generated the knockdown cell line with lentivirus and the cells were selected by puromysin for many...
04 April 2016 9,902 4 View
Read 11 answers by scientists with 7 recommendations from their colleagues to the question asked by Eman El-Abd on Apr 15, 2016
04 April 2016 8,225 10 View
I've come across a couple of papers now saying that high levels of vimentin increase the invasiveness of a cancer, I was wondering if anyone knew why?
04 April 2016 1,867 5 View
I generated iPS cells from urine cells of tumor patients. I want to induce tumor from iPS cells in vitro.I did specific differentiation and plated differentiated cells onto soft agar,but failed....
04 April 2016 3,995 3 View
04 April 2016 6,791 8 View
Hello, I need to use guinidine hydrochloride to clear any aggregates of apoA-I in my solution and then treat cells with it. What is a safe final concentration of GndHCL in the cell cultures?
27 March 2016 4,103 4 View
Hi All I am planning to study the molecular mechanism that link inflammation and cancer. I request you to advice me that whether two phytoconstituents(which share same biological property) can be...
26 March 2016 3,423 3 View
I am working on comparative study of cytokines secreted during cancer growth in cancer patients. I will extract the samples and would perform HPLC and OD for estimation of cytokines.
16 March 2016 4,246 3 View
I need a good reference about the cyotoxicity of ethanol in breast cancer cells. What´s the limit in ethanol concentration as vehicle to avoid interference in cytotoxic effect??
03 March 2016 2,939 2 View
immunohistochemistry WB ELISA
03 March 2016 9,830 4 View
Cancer is the second leading cause of death . About one-half of all men and one-third of all women in the US will develop cancer during their lifetimes. Today, millions of people are living with...
03 March 2016 7,187 19 View
03 March 2016 3,175 5 View
Hi, I'm looking into regulation of mitochondrial apoptosis by a novel compound. Gene expression results suggest it induces upregulation of both BNIP3 and BNIP3L and I'm considering looking at...
03 March 2016 2,220 1 View
03 March 2016 10,061 4 View
03 March 2016 3,444 4 View