Cancer Biology

A platform for people who work in the area of cancer biology | Contact experts in Cancer Biology to get answers

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Questions related to Cancer Biology

What are the best ways to measure ER Stress in humans?

What markers have you had success with in measuring ER Stress in human subjects?

02 February 2016 7,601 4 View

Anyone knows if have been classified some human cell lines of differentiated neuroblastoma? And if exist some natural remedies for this specific kind?

I know that exist some cell ines of indifferentiated neuroblastoma: RN-GA (stage III), GI-ME-N, SMS-KAN, SMS-KCN, SMS-SAN, NBL-S, NBL-W, KP-R-RT and some Others of poorly differentiated...

02 February 2016 7,698 1 View

Has anyone already used a Qiagen GeneReader and can comment on it for NGS?

Qiagen launched their NGS platform GeneReader in Nov 2015, i am curious if anyone can allready comment on it.

02 February 2016 8,928 3 View

Has anybody worked with U73122 (plc Inhibitor) from sigma?

I want to know the solubility and stability of U73122 (sigma aldrich).

02 February 2016 5,312 4 View

Can anybody suggest me a non toxic dye for tracking the solution while injecting in submucosal layer of caecum in mouse?

I am planning to develop a colorectal cancer model. For my pilot experiment i need to give an injection with vehicle in the subcutaneous layer of caecum. So i want to use some colored dye which...

02 February 2016 6,404 9 View

Does Live/dead assay with FDA/PI work for transwell cultures?

Hi! I would like to adapt the FDA/PI live/dead method to assess the viability of airways cells in PET membranes. Has anyone adopted this method on transwell cultures and can confirm it works....

02 February 2016 3,878 1 View

Why can we not obtain any results when using our telomere length assay kit?

We have employed the telomere length assay kit provided by Roche Company. Unfortunately, after trying several times, we could not obtain any result. DNA is first extracted from lymphocyte of CLL...

02 February 2016 9,467 3 View

Cancer cell cannibalism?

Would be "some RG Community members" so kind in providing me with articles (even if not top-ranged) about any type of chemicals able to induce ENTOSIS SPECIFICALLY in CANCER CELLS.Thank you very...

02 February 2016 8,180 4 View

IWP-2 inhibitor

Goodmorning everyone! I am using the IWP-2 inhibitor (IWP-2 inhibits the WNT pathway at the level of the pathway activator Porcupine) on melanoma cell line FO1 with a 20 µM concentration but when...

02 February 2016 7,702 4 View

Are all cancers truly immortal?

Subsets of cancer cells are known to reactivate telomerase and bypass senescence in many cases, but in order for a cancer to achieve lethality, is immortality of any cell subset actually...

02 February 2016 5,060 4 View

Hi! Someone can help me with SUMO1?

Hi!! Someone know best method to analyze SUMO1 by western blot? I tried different buffers to extract proteins, different loading buffers, different antibodies and doesnt work :S:S I'm getting mad...

02 February 2016 3,629 6 View

How can I prepare mucin gel with similar rheological properties to gastric mucus?

I am using pig gastric mucin type III (Sigma).

02 February 2016 1,547 3 View

Can anyone describe the mechanism of action of cancer?

Just give a general overview for this question.

02 February 2016 2,998 7 View

What can be the reason of not getting induction in expression hosts ??

I am trying to clone a gene and go for protein expression. But it is not getting induced . Although I checked my insert by digestion and then by sequencing before doing transformation in...

02 February 2016 7,359 6 View

I'm trying to stain cancer cells grown on gel. I'm not sure whether I need to fix my cells with 4%PFA or ethanol before staining?

I am trying to stain #ovarian cancer cells grown on gel with ethidium bromide and calcien. I am not too sure whether to fix my cells with 4% PFA or ethanol before staining. Any suggestions will be...

02 February 2016 5,345 5 View

What is the best method to analyze clonal architecture of cancer based on whole-exome or targe panel sequencing data?

I am looking into PhyloWGS. Can someone like me without bioinformatics background use it with ease?

02 February 2016 9,622 3 View

Has anyone used a gaussia luciferase construct instead of renilla in the Promega Dual Glo Luciferase Assay?

The Promega rep seemed to think that this had been successfully tested by customers, but said that Promega could not do in house testing because of licencing issues.

01 February 2016 9,606 1 View

Can I give spheroids in adherent culture of cancer stem cells in serum free medium?

I am working on cancer stem like cells, when I re-plated the cancer cells on collagen type I coated plate in serum free medium, after 12 days I observed adherent rounded shape cell aggregates...

30 January 2016 9,936 2 View

What do you think about the measurment of NFKB P65 subunit in serum ?

relation between seric levels of NFKB P65 and inflammation

27 January 2016 3,093 4 View

How can I extract tissue from frozen blocks (OCT compound) for protein analysis and ELISA?

I will be homogenizing the tissue in PBS with protease inhibitors and collecting supernatant for protein analysis and ELISA.

25 January 2016 9,704 3 View

What is the principle of estimation of nucleic acid?

I am working on Bioremediation.

20 January 2016 5,368 4 View

Anybody tried a CRISPR_based gene activation system?

I am curious how this system: lenti dCAS-VP64_Blastlenti sgRNA(MS2)_zeo backboneMS2-P65-HSF1_GFP Is this a good system? Thanks.

19 January 2016 2,964 2 View

Is ethanol a suitable solvent for Ibuprofen injection in rats??

I want to perform some biochemical experiments(cancer effect of ibuprofen on the liver) on ibuprofen, but these experiments require dissolve ibuprofen in a solvent for injection it to the rats....

17 January 2016 5,179 4 View

Is metabolic reprogramming common in IDH-intact and triple-negative gliomas?

TERT promoter mutations, IDH mutations and 1p/19q co-depletion are surely promising markers to classify clinically heterogeneous cases of glioma. Among Grade-IV gliomas patients, however, Figure1...

15 January 2016 7,666 1 View

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