We are planning to isolate glioblastoma cells for primary culture. After that we would like to establish a cell line from those primary cultures. I would like to ask the protocol for cell line establishment from primary cells. Thanks a lot!
We commercialize recombinant collagenases (COL G and COL H) available for your protocol.If you like more info please visit our website :www.abielbiotech.com or send an email at the following :[email protected] regards.Salvatore
After the treatment with collagenase, you should collect CD133+ cells (including both cancer initiating cells and daughter cells, but you can avoid the contamination with stromal cells) with flow cytometry, and then transfect SV40 large T antigen for immortalization.