A platform for people who work in the area of cancer biology | Contact experts in Cancer Biology to get answers
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Questions related to Cancer Biology
This aims to reproduce the most cited articles in cancer biology. From the ground up! Apparently they will be remaking organismal models from scratch. Who else is excited that this is finally...
12 December 2016 7,504 0 View
FGFR2 has isoforms IIIb and IIIc. I want to know the size in terms of the protein.
21 November 2016 1,784 1 View
Hello every one,I want to measure ribosome biogenesis in cancer cell line, please tell me what are the techniques used for this purpose, the best one? thank you :)
15 November 2016 586 5 View
p21KO mice: does anyone have access to them and would like to collaborate?
11 November 2016 7,525 2 View
We knock out a tumor suppressor gene in mouse to see whether it can promote the spontaneous development of intestine tumor. We were very happy as we observed some tumour-like lesions in the...
11 November 2016 486 9 View
11 November 2016 5,354 1 View
I am comparing normal mammary, primary mammary tumors and lung metastases of two different mouse strains to see any differences in the immune cell composition of these tissues. There are so many...
30 October 2016 811 4 View
I have anesthetized rats with urethane (2 g/kg) and I used 4-aminopyridine i.p to induce seizures. I saw all symptoms of freely moving rats with seizure from epileptic automatisms such as hair...
23 October 2016 6,095 2 View
Dear Colleague(s) I am about to clone a 200bp PCR fragment with restriction fragment overhangs Its been quite a few years since I performed this routine technique so I am wondering what are the...
10 October 2016 391 7 View
I performed the genotyping of breast cancer patients. I am willing to analyze the data for haplotypes analysis, cluster view and phylogenetic tree. In pyrosequencing the results comes in the form...
10 October 2016 6,713 4 View
I want to know how the blood perfusion values spreads in brain tumors, is that there is a blood flow distribution patterns in some types of brain tumors (for example the value in the contours is...
10 October 2016 7,711 2 View
I am searching for a questionnaire or a grid of questions that could help me to evaluate medical distorsions about cancer
10 October 2016 6,144 2 View
I am testing cytotoxicity of the molecules on the U251 and U87 glioblastoma cell lines. We did tests of the cytotoxicity with MTT and blue alamar, but since the molecules create radicals that...
10 October 2016 3,409 3 View
Hey guys, I've been struggling with quantifying miRNA with real-time PCR and the problem now i have is that i couldn't get consistent results. I tried to repeat the experiments literally two days...
10 October 2016 4,473 10 View
I am observing black dots or debris kind of stuff into culture dishes, fresh media, fresh cell revived but the problem persists. This does not make the media turbid but. This debris is less on the...
10 October 2016 6,348 10 View
Hi there; I need to evaluate an anti cancer vaccine against a human protein in murine models.So I should use human cell lines. Is there any convenient way to induce tumorogenesis in murine models...
10 October 2016 1,962 3 View
Hello, Is the TCR specificity of Jurkat cells (clone E6) known? Thanks,Vince
10 October 2016 6,005 0 View
How DHE and DCFDA detects ROS differently?
10 October 2016 4,643 4 View
We usually see that cancer progression is increased till death of a patient. Sometimes, this progression becomes fast after chemotherapy. As a result, patient dies due to synergistic effect of the...
10 October 2016 7,475 4 View
I am working on Ub-MDM2/MDM 2 assay kit. Any experience shared will be appreciated.
10 October 2016 1,754 1 View
I wanted to learn the best protocol of the siRNA transfection of mouse B cells , and most publications show the siRNA transfection of murine T cells ,
10 October 2016 7,255 1 View
Almost all of our cells are directly connected to the blood circulation. Thousands of our cells die every hour and spill their contents into the circulation to be cleared by the liver and...
10 October 2016 6,149 5 View
I am trying to induce apoptosis in 293T cells through FasL and TRAIL but has failed to show any apoptosis in cells. Why are 293T cells resistant to apoptosis?
10 October 2016 2,426 2 View
10 October 2016 9,919 2 View