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Questions related to Cancer Biology
Previously I tried with EBM-2 (cell: HUVEC), but I found that in cell free condition EBM-2 in presence on DCFDA (20uM) gives very high fluorescence. My plan is to replace EBM-2 with HBSS. But, I...
07 July 2016 1,265 5 View
Is there any difference in performing ChIP on suspension cells vs. adherent cells? I'm currently using Activemotif ChIP-IT Express Enzymatic Kit
07 July 2016 5,822 4 View
Compatible with MCF-7 breast cancer luminal-a epithelial cells.
07 July 2016 10,002 2 View
Please share protocol/reference paper
06 June 2016 3,730 4 View
Does Tevagrastim work at the same doses with same efficacy to mobilise haematopoietic stem and progenitor cells? Thank you
06 June 2016 4,252 1 View
I am on a trial to demonstrate the cell migration under knockdown conditions. Is anyone experienced with such assay for murine cardiomyocytes.HL1 (adult). Any suggestion will be highly...
06 June 2016 9,957 3 View
I have an expression vector carrying my gene of interest with T5 promoter and I want to transform it into BL21DE3 strain which is a T7 expression system. Just I want to know if doing so will not...
06 June 2016 2,166 3 View
In cancer cells, can autophagy inhibition leads genomic instability in order to promote cancer progression?? thank you for your help. please provide references if you will find
06 June 2016 325 2 View
1) Which one of the following has increased risk of becoming tumor ? A) Cells with shortest doubling time (often divide) and shortest life span. B) Cells with longest doubling time (rarely divide)...
06 June 2016 4,681 1 View
I am using whole mouse ovary for the qpcr analysis and I want to have a marker for each ovarian functional cellular structure. I chose markers for granulosa cells and theca cells but I am confused...
06 June 2016 1,371 1 View
if there is any gene specific carcinogen (che,bio,phy),we can initiate gene alteration specifically to tumor and later we can develop drug for it ... Carcinogen , Gene alteration, targeted drug
06 June 2016 5,668 4 View
Many confusing ranze of doses to follow in research articles. I would like to know two best doses of Doxorubicin for i.v. administration in xenograft mice. Frequency of administration will also be...
06 June 2016 6,226 2 View
I am interested to test the efficacy of my compound on pancreatic cancer cell lines. NCCS pune dont have them. Can someone share their freeze or help me to get one. ( except ATCC Obviously )
27 May 2016 2,950 2 View
How to product L929-conditioned medium for differentiate BMDM?
20 May 2016 1,002 2 View
I want to perform a time point analysis of expression of cytokines and signaling proteins in Jurkat T cells treated with a compound X. The confusion is whether these cells, being lymphomas have...
19 May 2016 3,529 5 View
Hello everyone, I need to know if the MDSCs cells can be isolated by magnetic beads, without using the flow cytometry to sort them, I found in an article a comparison between MACS and FACS...
16 May 2016 6,615 6 View
polyclonal antibody in the N-terminal part of Rpb1
13 May 2016 9,028 2 View
Our lab is currently testing the NTA (Nanoparticle Tracking Analysis) technique with the ZetaView from Particle Metrix. However we are really surprised how variable measurements are and how easy...
12 May 2016 7,107 1 View
05 May 2016 1,502 7 View
Dear all, I want to form amide bond between cysteamine and carboxylic acid. I have cysteamine hydrochloride and I was thinking about using CDI as coupling reagent (first stir CDI with acid and add...
05 May 2016 6,388 5 View
I'm working on mammospheres suspended in the media. I have no enough count to perform flow cytometry. I'm working on PCR for CSCs marker genes as well as the fluorescent markers. The problem is...
05 May 2016 4,060 6 View
05 May 2016 6,976 1 View
Would you please suggest an in house protocol of single emulsion solvent evaporation to follow for encapsulating Artesunate into PLGA polymer?
05 May 2016 6,911 2 View
05 May 2016 1,556 6 View