Electroporation conditions for primary retinal cell cultures (chick embryo)?

We are trying to transfect primary retinal cell cultures (chicken) with a pCAG (Addgene) plasmid but we have very low transfection efficiency (

07 August 2016 9,853 0 View

Calculation dilemma in a GH ELISA kit designed for media and plasma, Can you help us?

07 August 2016 3,105 6 View

In Response Surface Methodology is it mandatory to always start experimenting with first degree equation? Can one start with a second order one?

02 March 2016 3,798 2 View

What's the best way to shear DNA in a nuclear solution?

I've extracted fractioned protein lysates from adherent breast cancer cells. I've had good results with the cytosolic and mitochondrial fractions. However, the last fractioned obtained is a...

02 March 2016 5,078 4 View

Suggestions of methods for mitochondrial protein isolation? A good loading control for mitochondrial fraction?

Hi, I'm planning to prepare cell lysates to obtain mitochondrial and cytosolic protein fractions from adherent breast cancer cells. I've been looking into abcam's fractionation kit but was...

01 February 2016 4,090 3 View

Has anyone had any experience using the abcam Cell Fractionation Kit (ab109719)?

Hi. I'm interested in using this kit to obtain cytosolic and mitochondrial protein fractions from adherent breast cancer cells and was wondering if anyone had any experience (good or bad!) or...

01 February 2016 1,325 0 View

What's a suitable positive control for detection of PARP cleavage using western blotting?

I have good results showing the induction of PARP cleavage by different agents, but I need to include a positive control for publications. Any suggestions? Thanks!

31 December 2014 5,135 5 View

Basic electrochemistry question: how does the midpoint potential of an analyte affect its redox reactivity?

I've been involved in some analytical electrochemistry experiments to determine the reduction/redox potential of some compounds I'm working with. Cyclic voltammetry of two different analytes has...

10 November 2014 1,270 6 View

Did you collected centipedes?

I could collaborate by identifying scolopendromorph centipedes (Chilopoda: Scolopendromorpha).

01 January 1970 8,970 2 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View