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Questions related to Cancer Biology
Hi, we have recently started to use the CAM model and are at the moment rather successful in keeping embryos up to 14 days. The next step for us is to grow tumors on the cam and look at...
10 October 2016 3,856 0 View
I need to find out if my protein has binding sites available for intermolecular interaction with other proteins.
29 September 2016 8,708 3 View
Hello, I am working with endotoxin sensitive cells now and electroporation is quite toxic to them compared to an endotoxin free control. So I think I am going to clean up my existing plasmids...
09 September 2016 8,263 6 View
I would like to analysis the proteins involved in apoptotic pathway for target validation of docking studies.
09 September 2016 321 2 View
I'm using SPSS to draw a ROC Curve for some Tumor Suppressor genes. The problem is some of the curves are below the Standard Line (the diameter). The question is, how can I interpret these results...
09 September 2016 5,468 6 View
I have a plasmid with 9kb length, I am using DMSO since I heard it increases the efficiency. The PCR protocol I often use is as follows with various annealing temps such as 55, 60, and 65C; 95C 1...
09 September 2016 8,162 3 View
09 September 2016 5,284 4 View
All the methods i have seen so far are using EpCAM or CK staining. I want to use these CTCs for RNA isolation.
09 September 2016 6,959 6 View
the instruments required and techniques used in cancer laboratory
09 September 2016 4,943 3 View
Hello, Quick question: in vitro, could I get a T cell activation/proliferation if I stimulate human PBMC with anti-CD3+IL-2? thanks vince
09 September 2016 5,329 11 View
I'm doing a Knockdown experiment for a specific gene which has been associated with chromosome segregation. So I'm just wondering whether it will affect the efficacy of an anticancer drug if the...
09 September 2016 6,596 3 View
Hi, Referring to the above matter, I would like to know the contribution of the CAG repeats length to the risk of developing prostate cancer. In this regards, I am interested in counting...
09 September 2016 940 2 View
I am trying to knockdown basal level expression of my target microRNA by transfected Anti-Mir,miRNA Inhibitor, but I didn’t get significant level of knockdown result by real time PCR. Have any...
09 September 2016 2,116 1 View
Hi, I'm purifying Fab proteins but I have no time to finish it today so that I would like to continue the next day (I know i shouldn't but have no choice). Right now I'm centrifuging cell lysate...
09 September 2016 2,134 4 View
Read 1 answer by scientists with 1 recommendation from their colleagues to the question asked by Muhammad Waqas Usman Hingoro on Sep 28, 2016
09 September 2016 915 1 View
What are the most prevalent genes which mutated in prostate cancer ? What are the genes polymorphisms that show obviously in prostate cancer ?
09 September 2016 5,544 7 View
Hi, What should be the % of FBS added in the lower well of the cell invasion assay? I added the complete medium in the lower well which was prepared by 5% FBS and 0.1% ITS as growth supplement. ...
09 September 2016 8,378 5 View
The incubation period of the disease in which the patient has no external symptoms and the disease does not affect a person's life.
09 September 2016 6,333 3 View
why some results are quite different between the presence and absence of serum? which component in serum effects the response of cells?
09 September 2016 8,070 4 View
I want to know the detailed mechanism of cis platin and carboplatin with references please
09 September 2016 2,070 2 View
Hi, During our experiments we noticed that nude mice orthotopically grafted with GBM, develop cutaneous pustules when treated with TMZ (schedule: 1 week on and 2 weeks off for 6 cycles). We...
09 September 2016 8,421 5 View
So many companies and technologies to choose from, I would need some advice. I want to transfect glioblastoma cells with different miRNAs and measure cell viability.
09 September 2016 951 3 View
Picrosirius red staining for collagen can be used to distinguish between type I and III collagen under polarized light. But under regular light of a bright-field microscrope, does the red staining...
09 September 2016 1,930 7 View
can I have the reference as well?
09 September 2016 3,554 3 View