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Questions related to Cancer Biology
The spheroids are made of MDA-MB-231 breast cancer cells which are themselves producing GFP-fusion proteins. Only very portions of the periphery can be seen by the confocal microscope, whereas the...
09 September 2016 605 3 View
I have prepared Plasmid Encapsulated PLGA nanoparticle. How should i treat the HEK293T cells or any other cells with these nanoparticles to get the plasmid inserted into the cells??
09 September 2016 2,487 1 View
Hi, i am currently looking for an experiment alternative to southern blot. I heard that pcr based assays give results quickly ( let's say within few hours). I want to perform sybr green relied pcr...
09 September 2016 4,147 3 View
Hello, I have transplanted C57bl6 mice with oncogenes. When the mice become sick, i would like to isolate spleenocytes, bone marrow, peripheral blood and freeze it for future secondary...
09 September 2016 7,371 2 View
I want to analyse few plant sample for my further studies. does any one have this facility please suggest me so that i can come & do my work.
09 September 2016 3,070 5 View
Can anyone suggest which software or strategy is appropriate to find how binds small molecule to protein? for example some inhibitor that prohibits aggregation of some protein, how to predict...
09 September 2016 3,896 2 View
Picrosirius red staining for collagen can be used to distinguish between type I and III collagen under polarized light. But under regular light of a bright-field microscrope, does the red staining...
02 September 2016 9,569 8 View
single cell genomics
08 August 2016 6,137 1 View
Hello, Do any one know how TCGA database works? any tutorial to show ho to use it and interpret the data? Also, any idea about any database website that shows the expression level of specific...
08 August 2016 3,725 2 View
Which depression model for rats do you prefer? What is the best way to induce depression in rats? We would like to start some projects in this field and we do not have any experience....
08 August 2016 2,628 3 View
I have some parraffin blocks with cells extracted from gastric cancer patient and healthy patients. So far, I did not find a marker which was exclusively expressed in gastric cancer. Does anybody...
08 August 2016 7,141 3 View
It is known from evidence that more than 90% of cancer cervix are attributed to Human Papilloma virus. Infection occurs due to sexual exposure. What exactly is the source and reservoir of infection?
08 August 2016 2,528 2 View
I want to develop Tamoxifen resistant cells for my research but getting confused with different protocol used.
08 August 2016 4,235 8 View
I want to know if there has been any study about how permeable can be the capsule that surrounds tumors. what is it made of?
08 August 2016 9,444 19 View
I measure tumors in thigh (mouse). I usually use a vernier but i find it somehow subjective. Tumors are not always perfect round wich makes it difficult, is there something else that might be...
08 August 2016 8,102 7 View
Hi everyone, I am looking for informations about interferon-gamma synthesis, especially I was wondering if it could be synthetized by other cells than immune cells. Does anyone heard about that...
08 August 2016 9,008 3 View
Dear Colleagues, I am searching high-impacted scientific journals giving a "crystal-clear" response to my question here above, but as yet unsuccessfully. If anyone has such articles, I would be...
08 August 2016 9,466 4 View
Tumor heterogeneity and metastasis correlate with the clinical aggressiveness of cancer. What is the future direction in this field of science? Please share it?
07 July 2016 10,052 4 View
The most devastating aspect of cancer is the emergence of metastases in organs distant from the primary tumor. Knowing the molecular mechanisms of metastasis is one of the most important issues in...
07 July 2016 1,837 4 View
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07 July 2016 2,308 3 View
Hello everyone, I am working on expressing a his-tagged 27.7 kDa protein (with 250 amino acids and 7 Cys residues) in BL 21 (DE3) cells using the pET15b plasmid. Unfortunately, getting a good...
07 July 2016 4,804 4 View
Who knows what's the experience of FBS-exosome?
07 July 2016 3,276 6 View
I've been trying to isolate extracellular vesicles on an optiprep gradient in 5-ml tubes. I've tried 2 and 12 h centrifugation and in both cases, the fractions seemed to be mixed after...
07 July 2016 3,343 5 View
Hi friends do you like to participate in national symposium on current research in cancer biology & therapy? Here is the attachment and links please go through it. It’s a nice one.
07 July 2016 8,203 0 View