Hello, I need to use guinidine hydrochloride to clear any aggregates of apoA-I in my solution and then treat cells with it. What is a safe final concentration of GndHCL in the cell cultures?
Upto 20 mM seems to be acceptable at least in L929 cells, without affecting viability ( http://jvi.asm.org/content/81/9/4572/F1.expansion.html ). Obviously, better to have GmCl alone controls. Hope it helps.