Does anybody know whether CFSE is retained in cell nuclei after treatment of cells with pepsin solution in HCl?
We are interested in CD8+T lymphocytes form pancreas cancer patients. Is it possible to find a pancreatic cancer cell line with HLA class I molecules that could be recognised by CD8+T lymphocytes...
15 December 2023 4,173 1 View
We were culturing murine B16 melanoma cells and were trying to prepare a cell suspension after detachment of cells using trypsin. However, cell clumps were formed. Did we make something wrong? I...
20 March 2023 6,023 4 View
What is the most suitable interpolation method (IDW, spline, kriging, natural neigbours) for evenly and dense distributed points (attached picture .jpg)? and if you recommend any can you explain...
06 January 2020 6,958 9 View
On CD4+ T lymphocytes CD28 functions as co-stimulatory molecule during antigen presentation by dendritic cells. But CD8+ cytotoxic cells apparently do not need CD28 for killing their targets..?
05 January 2019 587 5 View
The Research Council of Lithuania (LMT) and Japan Society for the Promotion of Science (JSPS) are supporting the development of strong bilateral research and dissemination projects across the...
01 January 1970 262 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View