If you mean staining sections on Synaptek large slot grids, it is not much different from staining normal square mesh grids. I used to make my own Formvar-coated slot grids and place the sections on the Formvar of the slot grids. Synaptek grids are thicker than normal mesh grids, which aids in the staining procedures ( less physical bending/damage). I never found commercial-coated grids to be very good; the support films tended to break. And always blot the grids from the sides, not face-on.
Thank you. I"ve read that you can stain on the grids, but we want to put stained tissue on the grid, and add heavier metals than osmium tetroxide and uranyl acetate, to try to increase contrast. Thinking of lining the grids up in the hood, adding a drop of lead aspartate staining solution and then see if it can take that. Or other metals?
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