I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but not the lung. I had to perform up to 10 repetitions for each sample. All samples weigh 50 gr. Now I have to process skin and heart tissues, which in theory have greater mechanical resistance. What do you recommend? Should I change the matrix or perform more repetitions or do a pre-maseration?
All this, taking into account that I must work in cold, since I intend to detect the levels of tissue factor and beta actin in the lysed tissues.
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