Has anyone used the TUNEL assay for 3D bio-printed models cell cultures?

17 August 2024 0 4K Report

Has anyone used the TUNEL assay for apoptosis detection in 3D cell cultures, such as 3D bio-printed models?

I'm planning to construct 3D bio-printed models from U87 glioblastoma cells and study the apoptosis assay using TUNEL assay in these 3D models when treated with drug and U87 exosomes. Is it possible? Do I need to do sectioning first?

If so, I would appreciate any insights or protocols you could share. Thank you!

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