For an Anti-p24 (HIV) Ag capture ELISA, I am getting low OD readings for a 20-90pg/mL range. What should my primary Ab working concentration be?

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Gertrudis Rojas

I dont understand properly how is your ELISA performed. the results are also unclear for me. could you add more details?

Jody D Berry

Yes also if you are using commercially available mAbs we may be familiar with?

Chad Coarsey

If it helps anyone who is wondering for a plate ELISA, regardless of type should be tested with ranges of anti-p24 from 1-10ug/mL