Can Copper and Cobalt ions improve hyaluronic acid uptake by mesenchymal stem cell line (C3H10T1/2)?

More Oleksandra Skorobohatko's questions See All

Can I use gel and samples for Western Blot prepared in advance?

Hi everyone! I need to perform Western Blot but, unfortunately, my schedule allows me to be in the lab Monday and Wednesday (skipping Tuesday). Can I prepare gel and samples on Monday and run the...

29 October 2023 5,428 0 View

Any research on mutual understanding between mentally ill patients?

Could you please share any research on interaction, mutual understanding, relationships, etc., between people with mental disorders? I am interested whether there can be situations when a mentally...

11 December 2022 9,413 10 View

How do you synthesize a peptide?

Dear all, I need to synthetize a peptide for antibody production, since I had a hard time to purify one very stubborn protein. Please, help me to figure out the right strategy how synthesize a...

02 February 2022 3,880 5 View

What does it mean if the Ni-NTA resin has turned into a gel in the column?

Dear All , I was purifying the proteins, following ProBond™ Purification System protocol (denaturing conditions) and noticed that Ni-NTA resin turned into something like gel structure inside the...

02 December 2021 6,196 3 View

What is the most effective way to elute a his tagged protein from the beads?

Dear All, I'm purifying some HIS tag protein in denaturing conditions and it seems that it does not elute from the beads efficiently (checked the gel).. Do you have any suggestions what is the...

24 October 2021 4,969 16 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,771 2 View

How to combine three-part variable into one variable?

I asked the same respondents about how much luck, their own efforts and connections they need to achieve Goal # 1, # 2, # 3, # 4. (first group). The second group had the same questions and the...

09 August 2020 3,740 5 View

Can I apply cholesterol dissolved in DMSO to activate ion channels?

I do patch clamp (and Ca2+ imaging) and usually apply DAGs-DMSO in external buffer through the perfusion. Now I want to see if my ion channels will respond to cholesterol. I prepared...

05 August 2020 206 0 View

Cross-cultural adaptation of a questionnaire: what are the requirements for a translator?

Hello! I am planning cross-cultural study in psychology. I've read various articles, but I can’t understand what are the requirements for translators? I’m at the stage one - translating the...

27 February 2020 2,518 11 View

How to calculate Cronbach alpha using SPSS, if 2 items are “inverse”?

Update I have a scale (12 items) I go to Analysis -> Scale -> Reliability analysis and get my Cronbach alpha (0,5) BUT 2 of my items are «inverse». If I recode this two items as it was not inverse...

23 February 2020 5,508 7 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,489 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,524 4 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,781 3 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 639 2 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 665 4 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,621 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 500 2 View

Chinaza Godswill Awuchi

Check https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829098/

Also check https://pubmed.ncbi.nlm.nih.gov/26915044/

Alexandr Chernov

Article Applications of Metals for Bone Regeneration

Together with iron and zinc, copper is one of the most important metals for ... loaded with hyaluronic acid–gelatin hydrogel for bone regeneration.

Kiprotich Kiptum

Hello Kindly look at this paper.

https://www.mdpi.com/1999-4923/13/3/432/pdf