Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to combine three-part variable into one variable?

I asked the same respondents about how much luck, their own efforts and connections they need to achieve Goal # 1, # 2, # 3, # 4. (first group). The second group had the same questions and the...

09 August 2020 3,502 5 View

Cross-cultural adaptation of a questionnaire: what are the requirements for a translator?

27 February 2020 2,289 11 View

No title

23 February 2020 5,145 7 View

After adding stop solution (sulphuric acid) in reaction mixture of HRP assay with TMB, yellow color becomes brighter. What is the mechanism?

Yellow colour, before the reaction is stopped, is due to the formation of oxidized TMB, which isTMB(2+). The reaction is stopped by degrading HRP, but are there any further oxidations happening?...

13 December 2019 269 3 View

What are advantages and disadvantages of inducible promotors, namely of positive and negative regulation?

When we have a positive regulation the control is tight, so we have low background expression under non-induced conditions. Negative regulation can not be fully controlled, but as far as I...

25 January 2019 2,007 5 View

Are there any known hybrids in the complex of Lacerta viridis?

Can species of Lacerta viridis complex hybridize at all? And if yes, then can Lacerta viridis hybridize with Lacerta bilineata, Lacerta strigata, Lacerta trilineata?Can you recommend literature on...

03 September 2017 1,424 4 View

Has anyone had a problem with HEK293 insensitivity to Carbachol?

Hi everybody! Would be very thankful if someone can help with a small problem! Suddenly HEK293 became insensitive to Carbachol stimulation in FURA experiments. With application of Carbachol no...

23 November 2015 1,873 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View