Multiphysics simulation of low temperature plasma, what is the best approach?

Any thoughts on the best approach or framework to adopt here? also what is the best computational environment to use for this modelling task, ANSYS or COMSOL?

17 December 2019 3,058 3 View

Are there any open source Monte Carlo ray tracing software available for download?

Using ray tracing software to model a luminescent solar concentrator.

19 February 2019 6,861 1 View

How do I control for confounders in Minitab 17?

Dear all, I am currently investigating the effect of several predictor variables (continuous and categorical) and how they impact the response variable (continuous). The thing is, I am confused as...

07 April 2017 5,538 2 View

What post hoc test(s) are available for Mood Median test?

Dear all, I conducted a Mood Median test and determined a statistically significant difference between the medians of the 3 groups of data being analysed. However, I am wondering what post hoc...

21 February 2017 5,109 13 View

Can hoescht stain rDNA in nucleolus?

Hi, I am wondering if hoescht or dapi can stain rDNA in nucleolous. Normally they just gives a hole in the nucleus indicating nucleolus. Or if any one has a good way to visualise nucleolus...

14 November 2016 7,929 3 View

Other than Stepwise Analysis, alternatives to find significant predictors?

28 July 2016 2,278 3 View

What is the percentage knockdown needed for a valid siRNA experiment?

Utilizing western blot analysis, I was able to knockdown NRF2 by 55%. Does it need to be closer to 20% or less?

22 September 2015 9,146 7 View

Is a species niche the habitat that it does or could potentially inhabit, or is it a model describing that set of habitats?

03 December 2014 4,341 8 View

Zotero Vs EndNote ??

What are folks mainly using these days?

01 January 1970 3,510 12 View

Can i do harmonic response with these information in Ansys?

Hi guys, Question : can i obtain information from this picture to do harmonic response in Ansys ? if not what are the lack information ? sorry cause i got this from my researcher and i'm new to...

01 January 1970 2,747 1 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

Why there are two peaks while using the mie plot of Silver nanoparticle?

Hi, I am trying to learn the theoretical modelling of silver nanoparticle using the Mie scattering theory. After that, I discovered a software named Mieplot developed by Philip Laven, where it...

25 February 2021 7,493 6 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View