I prepared a 5X of M9 minimal salt and 1000X elemental trace and mixed these 2 solutions to produce carbon-free minimal salt media. Biphenyl was used as a carbon source but was prepped separately with polar aprotic solvents such as acetonitrile, acetone, and DMSO. The concentration of biphenyl was 100 mg/mL.
The CFMM was mixed with the following volumes and reagents, and this would give me approximately 0.5% of Biphenyl in CFMM.
- 20 mL of 5X M9 minimal salt
- 10 μl of 100X or 1000X elemental trace
- 500 μl of 100 mg/mL Biphenyl
- 79.4 mL of autoclaved Milli-Q water.
The problem that I've encountered when I added 100 mg/mL of biphenyl (dissolved acetonitrile/acetone/DMSO) into the carbon-free minimal salt media, it (biphenyl) flocs and does not get homogenized with the CFMM (Carbon-free minimal salt media).
I've tried using milli-Q water as a solvent and heat the biphenyl to its melting point of 69.2 Celcius, and the result does not change when I added it to the CFMM.
What can I do to helps Biphenyl dissolute and homogenize with CFMM?
Hao Chau:
Do you have to use biphenyl? Could you use something else a little more water soluble?
One question already asked is do you have to use biphenyl. Another question is do you have to use water. You did not indicate what you are trying to do. I wonder, because you mentioned PCBs in your response above, but biphenyl is not a PCB (as you know). Perhaps you could use a hydroxylated form of biphenyl with one or more hydrogen atoms substituted by -OH groups. OH groups may give the possibility of hydrogen bond formation with water molecules.
Just like Michael said, maybe use 4,4'-Dihydroxybiphenyl which has better water solubility.
- Badges
- Science topic
- Similar topics
- Chemistry
- Biochemistry