What effect size should i use for a meta-analysis when study data violate normality assumptions?

I'm looking for advice regarding what effect size to use for meta-analysis. Our question is whether the number of individuals in a group affects the per-capita productivity of that group. Does...

10 February 2021 5,510 8 View

Having trouble with qPCR amplification plots. My curve's never seem to reach a plateau. Any ideas?

The parameters of the curve all seem acceptable, typically reactions results are above 0.9852, -3.1 to -3.6 slope, and 90-110% reaction efficiency. The results seem to be what I would expect from...

04 February 2021 1,138 3 View

What are the conflicts between EIA and environmental engineers and lawyers?

Environmental Impact Assessment (EIA) does not live up to the expectation of environmental lawyers and environmental engineers. In your own opinion, how can the EIA process therefore be made more...

03 February 2021 1,329 6 View

Multicloning in pRMC2?

Hi all, I want to express several genes in S.aureus by using the pRMC2 expression vector. I want to try to subclone some genes already cloned into pRMC2 (and their own tetO promoter) and put them...

01 February 2021 5,108 3 View

How to convert a domestic microwave assisted synthesis for dedicated instrumentation?

Hello, I just dived into the topic of microwave assisted synthesis and found a literature I would like to reproduce. The problem is in the literature, a domestic microwave oven is used which we...

27 January 2021 4,788 4 View

I am trying to run a simulation on LS-Dyna and I am facing the error below with Nodal rigid constraints. Can anyone tell how to fix it?

*** Error 20393 (STR+393) Nodal rigid body ID 85000010 contains node ID 83500180 already used in a rigid body/nodal rigid body definition.

20 January 2021 2,019 1 View

Can you give me an example of a faulty or egregious environmental claim that is NOT related to climate change?

I am doing lesson plans and am looking for examples of environmental claims that do not stand up to close scrutiny. There are more than enough examples of this related to climate change...so no...

18 January 2021 4,661 7 View

How do you improve retroviral expression from PG13 cells?

I have been trying to express a gene (GFP) in a retroviral system by transfecting human Phoenix-ECO cells, and transducing murine PG13 cells with the supernatant. I then sort GFP-positive PG13...

18 January 2021 476 1 View

Numerical taxonomy with SPSS?

Hi everyone, I was wondering about the possibilities of performing numerical taxonomy with SPSS software. I would be very thankful to recieve advice!! For now I have been reading about...

12 January 2021 5,549 3 View

DFT Optical calculation and plotting of Real and imaginary Permittivity.??

Dear DFT Community, i have calculated Frequency dependent optical permittivity of Au using (VASP) my INCAR tag includes LOPTICS=.TURE., my OUTCAR has expected frequency dependent IMAGINARY...

30 December 2020 9,543 9 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Is there a fast method matching citations to bibliography (manually typed references)?

I need to be able to match in-text citations to a reference list and back again in very large documents (100+ pages) WITHOUT the use of referencing software like Endnote. my method is to type the...

27 February 2021 9,848 1 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

What are the latest topics in strategic management area, esp those related to Quantum managment?

I am trying to involve science , in particular Physics, into Strategic Management studies for my master thesis . There are a number of papers discussing this topic but in such a fast changing...

13 February 2021 891 5 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

How to convert isotrophic Deriche filter into anistrophic?

12 February 2021 5,889 6 View

Cell culture compatible glue/sealant?

Hello We are looking for a glue /sealant to fix and secure some glass parts of a live cell imaging chamber. It has to be biologically inert because it may come in contact with the medium, and...

01 February 2021 6,886 3 View

Can Cas13a protein directly purify from PAGE gel, please suggest me the method?

I am willing to Cas13 in vitro. Can it be directly purified from PAGE gel, please suggest me the method.

19 January 2021 1,136 3 View

Can data collected through participant observation be interpreted through Interpretative phenomenology?

I have just started to read about phenomenology and hence quite a beginner. I have observed hospital interactions between doctors and patients, noted down the conversations, added the non-verbal...

14 January 2021 3,452 3 View

Johnson et al., 2006 Geology of South Africa?

I am wondering if there is a digital (PDF) version of this book available or if anyone is willing to share this work. It is no longer available from GSSA (Geo Soc. S. Afr) nor does my library hold...

13 January 2021 5,571 2 View