Dear Colleagues,
I am currently studying the recruitment of monocytes to muscle injury in mice using flow cytometry. I am utilizing CD45 and those 2 markers: Ly6C and CCR2 (as markers for pro-inflammatory macrophages). During my analysis, I noticed that some monocyte-derived macrophages are Ly6C⁺ CCR2⁺, while others are Ly6C⁺ CCR2⁻. Could anyone explain the difference between these two populations?
Thank you
Ly6C⁺ CCR2⁺ inflammatory monocytes are typically involved in acute inflammatory responses and can differentiate into macrophages or dendritic cells at the site of inflammation. CCR2 is crucial for their migration from the bone marrow to sites of inflammation.
Ly6C⁺ CCR2⁻ monocytes are thought to play a role in tissue repair and homeostasis. They can also accumulate at sites of inflammation but are more associated with resolving inflammation and promoting tissue repair
Dr. Sabine Strehl,
Thank you very much for your response. I understand now that Ly6C⁺ CCR2⁺, typically known as pro-inflammatory macrophages, transition to Ly6C⁺ CCR2⁻, at which point they start exhibiting pro-repair characteristics.
Could there be a possibility that the Ly6C⁺ CCR2⁻ macrophages are resident or memory macrophages rather than transitioned from the Ly6C⁺ CCR2⁺ population?
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